IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25520
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Canine (95%), Porcine (92%). Backed by our 100% Guarantee.
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1982 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human IGF-II/IGF2
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%), Sheep (87%), Bovine (87%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free (NBP3-25520) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
Immunocytochemistry/Immunofluorescence: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - IGF2 antibody [HL1982] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2 stained by IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:500. Blue: Fluoroshield with DAPI .
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] -
Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
Application
Recommended Usage
ELISA
Assay dependent
Immunocytochemistry/ Immunofluorescence
Assay dependent
Sandwich ELISA
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IGF-II/IGF2
Long Name
Insulin-like Growth Factor II
Alternate Names
C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974
Gene Symbol
IGF2
Additional IGF-II/IGF2 Products
Product Documents for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
Product Specific Notices for IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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