IGF-I/IGF-1 Antibody (SPM406)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34361
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Rabbit
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM406
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Purified human IGF-I/IGF-1 protein (Uniprot: P05019)
Localization
Cytoplasmic (Secreted)
Specificity
This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for IGF-I/IGF-1 Antibody (SPM406)
Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (SPM406) [NBP2-34361] -
CD11c+ microglia expressed higher phagocytic genes, myelin supportive genes, and lipid metabolism associated genes than CD11c− microglia.A Gating strategy and the fluorescence minus one (FMO) sample to identify CD11c− and CD11c+ microglia on tMCAO 14d. qPCR for CD11c (B), Csf1 (C), Igf-1 (D), Spp1 (E), CD68 (F), and Axl (G) mRNA expression in CD11c− and CD11c+ microglia, respectively. n ≥ 3 repeats. The results of qPCR for Abca1 (H), Abcg1 (I), Apoe (J), Apoc1 (K), and Lpl (L) mRNA expression in CD11c− and CD11c+ microglia, respectively, on the 7th Day post-stroke. n = 5 repeats. M Representative images of CD11c (red), CD68 (orange), IGF-1 (gray), and MBP (green) immunostaining on the sham group and 7th Day after tMCAO. Scale bars, 100 μm. *p < 0.05, **p < 0.01, and ***p < 0.001. B–L Compared to CD11c- group. Values were mean +/- SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36828819), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for IGF-I/IGF-1 Antibody (SPM406)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
0.5-1.0ug/ml
Application Notes
ELISA: For coating, purchase antibody without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT.
In-vitro Neutralization of Biological Activity of IGF-I/IGF-1: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT.
In-vitro Neutralization of Biological Activity of IGF-I/IGF-1: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: IGF-I/IGF-1
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
UniProt
Additional IGF-I/IGF-1 Products
Product Documents for IGF-I/IGF-1 Antibody (SPM406)
Product Specific Notices for IGF-I/IGF-1 Antibody (SPM406)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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