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IFN-gamma Antibody (JM10-10)

Catalog # NBP2-66900 | Novus Biologicals a Bio-Techne Brand
Recombinant Monoclonal Antibody.
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NBP2-66900

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM10-10

Concentration

1 mg/ml

Product Summary for IFN-gamma Antibody (JM10-10)

Immunogen

Synthetic peptide within N-terminal human IFN-gamma. (SwissProt: P01579 Human; SwissProt: P01580 Mouse; SwissProt: P01581 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IFN-gamma Antibody (JM10-10)

Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Human liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Analysis of Hela cells with Interferon gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: IFN-gamma Antibody (JM10-10) [NBP2-66900] - Mouse liver tissue using anti-Interferon gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for IFN-gamma Antibody (JM10-10)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500
Application Notes
Use in ICC/IF reported in scientific literature (PMID:33491667).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 6 publications using NBP2-66900 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFN-gamma

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Gene Symbol

IFNG

Product Documents for IFN-gamma Antibody (JM10-10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IFN-gamma Antibody (JM10-10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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