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ID4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56322

Novus Biologicals, part of Bio-Techne
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NBP2-56322-25ul
NBP2-56322

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PLTALNTDPAGAVNKQGDSILCR

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:31828317).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ID4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ID4 Antibody [NBP2-56322]

Immunocytochemistry/ Immunofluorescence: ID4 Antibody [NBP2-56322]

Immunocytochemistry/Immunofluorescence: ID4 Antibody [NBP2-56322] - Staining of human cell line HEK 293 shows localization to nucleus. Antibody staining is shown in green.
ID4 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ID4 Antibody - BSA Free [NBP2-56322]

Chromatin Immunoprecipitation-exo-Seq: ID4 Antibody - BSA Free [NBP2-56322]

ChIP-Exo-Seq composite graph for Anti-ID4 (NBP2-56322) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for ID4 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ID4

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes ina number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo-and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain theHLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA andtranscriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 (PubMed7665172)).(supplied by OMIM)

Long Name

Inhibitor of DNA Binding 4

Alternate Names

Idb4

Gene Symbol

ID4

Additional ID4 Products

Product Documents for ID4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ID4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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