Humanin Antibody - BSA Free

Western Blot: Humanin Antibody [NB100-56877]

Western Blot: Humanin Antibody [NB100-56877] - Analysis of Humanin in (A) recombinant fusion protein containing amino acids 1-24 and (B) fusion partner without these amino acids, using Humanin antibody at 1:2000.

Immunohistochemistry-Paraffin: Humanin Antibody [NB100-56877]

Immunohistochemistry-Paraffin: Humanin Antibody [NB100-56877] - Analysis Humanin in human testis at 1:100.

Immunocytochemistry/ Immunofluorescence: Humanin Antibody - BSA Free [NB100-56877] -

TNF suppressed humanin expression in human growth plate tissue specimens (n=6) obtained from 2 children or human chondrocytes. (A, B) Quantitative analysis of humanin staining (yellow arrows), calculated as number of positive cells per mm2. (C) Relative expression of humanin assessed by qPCR in HCS-2/8 cell line treated for 72 hours with TNF at 10, 30,100, 300 ng/ml concentrations (n=3-4). (D) Quantification of humanin expression by immunofluorescence in HCS-2/8 cells treated with TNF (100 ng/ml). (E) Representative images of humanin staining (red) in HCS-2/8 cells treated with TNF and untreated controls. Error bars indicate mean +/- SE. Students t-test was used to analyze differences between groups. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fendo.2023.1142310/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.