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Human Wnt‑2 Antibody

Catalog # AF3464 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity



Immunohistochemistry, Western Blot



Antibody Source

Polyclonal Goat IgG

Product Summary for Human Wnt‑2 Antibody


E. coli-derived recombinant human Wnt‑2
Thr33-Glu75, Leu222-Arg292
Accession # P09544


Detects human Wnt-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Wnt‑7a and rhWnt‑7b is observed.







Scientific Data Images for Human Wnt‑2 Antibody

Detection of Human Wnt-2 antibody by Western Blot.

Detection of Human Wnt‑2 by Western Blot.

Western blot shows lysates of human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3464) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Wnt‑2 antibody in Human Stomach Cancer Tissue

Wnt‑2 in Human Stomach Cancer Tissue

Wnt‑2 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using 10 µg/mL Goat Anti-Human Wnt‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3464) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Wnt‑2 Antibody

Recommended Usage


5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: Human brain (hypothalamus) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Antigen Affinity-purified


Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-2

Human Wnt-2 is a 35 kDa, secreted, glycosylated member of the Wnt family of developmental proteins. It is considered a class 1 Wnt based on its strong transforming activity on C57MG cells. Human Wnt-2 is synthesized as a 360 amino acid (aa) precursor that contains a 335 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature human Wnt-2 is 96% aa identical to mature mouse and rat Wnt-2. Wnt-2 is produced by mammary myoepithelial cells, endometrial epithelial cells, and breast fibroblasts.

Long Name

Wingless-type MMTV Integration Site Family Member 2

Alternate Names

INT1L1, IRP, Wnt2

Entrez Gene IDs

7472 (Human); 22413 (Mouse); 114487 (Rat)

Gene Symbol



Product Documents for Human Wnt‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Wnt‑2 Antibody

For research use only

Reconstitution Calculator

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