Human Wnt‑2 Antibody
Catalog # AF3464 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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Product Summary for Human Wnt‑2 Antibody
Immunogen
Thr33-Glu75, Leu222-Arg292
Accession # P09544
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Wnt‑2 Antibody
Detection of Human Wnt‑2 by Western Blot.
Western blot shows lysates of human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3464) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Wnt‑2 in Human Stomach Cancer Tissue
Wnt‑2 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using 10 µg/mL Goat Anti-Human Wnt‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3464) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Wnt‑2 Antibody
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human stomach cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
Sample: Human brain (hypothalamus) tissue
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-2
Human Wnt-2 is a 35 kDa, secreted, glycosylated member of the Wnt family of developmental proteins. It is considered a class 1 Wnt based on its strong transforming activity on C57MG cells. Human Wnt-2 is synthesized as a 360 amino acid (aa) precursor that contains a 335 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature human Wnt-2 is 96% aa identical to mature mouse and rat Wnt-2. Wnt-2 is produced by mammary myoepithelial cells, endometrial epithelial cells, and breast fibroblasts.
Product Documents for Human Wnt‑2 Antibody
Product Specific Notices for Human Wnt‑2 Antibody
For research use only
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.