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Human VEGF/PlGF Heterodimer Antibody

Catalog # MAB297 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity



Western Blot



Antibody Source

Monoclonal Mouse IgG2A Clone # 146213

Product Summary for Human VEGF/PlGF Heterodimer Antibody


E. coli-derived recombinant human VEGF/PlGF heterodimer
Ala27-Arg191 (VEGF) & Ala21-Arg149 (PIGF)
Accession # AAM03108 (VEGF) & AAD30179 (PIGF)


Detects human VEGF/PlGF heterodimer in direct ELISAs and Western blots. This antibody does not detect either subunit alone. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) VEGF121, rhVEGF165, rhVEGF206, rhCTGF, rhLDGF, rhPlGF, rhVEGF‑B186, rhVEGF‑C, rhVEGF‑D, rhPDGF, rmVEGF115, rmVEGF120, rmVEGF164, rmPlGF‑2, rrVEGF164, or rzfVEGF.







Applications for Human VEGF/PlGF Heterodimer Antibody

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human VEGF/PlGF Heterodimer (Catalog # 297-VP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant


Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF/PlGF Heterodimer

The disulfide-linked homodimeric proteins vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) are both members of the VEGF family of growth factors. VEGF and PlGF proteins share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. E. coli-expressed monomers of PlGF and VEGF can be refolded in vitro to form PlGF/VEGF heterodimers. The purified recombinant VEGF/PlGF heterodimers and VEGF homodimers have potent mitogenic effects on endothelial cells. However, VEGF/PlGF heterodimers display approximately 50-fold less mitogenic activity than VEGF homodimers. In contrast, PlGF homodimers have little or no mitogenic effect on endothelial cells. The presence of natural VEGF/PlGF heterodimers in the conditioned media of a number of human tumor cell lines has been demonstrated using a VEGF/PlGF heterodimer specific ELISA.

Long Name

Vascular Endothelial Growth Factor/Placenta Growth Factor Heterodimer

Alternate Names

VEGF/PlGF Heterodimer

Product Documents for Human VEGF/PlGF Heterodimer Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VEGF/PlGF Heterodimer Antibody

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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