Human VEGF_165b Antibody

Name: Human VEGF 165b Antibody
Brand: R&D Systems
SKU: MAB3045
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB3045

Citations (10)

(2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	KLH-conjugated human VEGF_165b synthetic peptide TCRSLTRKD Accession # AAL27435
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 56-1
Specificity:	Detects human VEGF_165b in direct ELISAs and Western blots. Recognizes an epitope within a 9 amino acid sequence at the C-terminus of human VEGF_165b. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) VEGF₂₀₆, rhVEGF-B₁₆₇, rhVEGF-B₁₈₆, rhVEGF-C, or rhVEGF-D is observed.

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Applications for Human VEGF_165b Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human VEGF_165b (Catalog # 3045-VE)
Immunohistochemistry	Bates, D.O. et al. (2006) Clinical Sci. 110:575.
Immunofluorescence	Woolard, J. et al. (2004) Canc. Res. 64:7822.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human VEGF_165b Antibody

	Immunohistochemistry VEGF_165bin Human Placenta. VEGF_165bwas detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human VEGF_165bMonoclonal Antibody (Catalog # MAB3045) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: VEGF

VEGF_165b is the protein resulting from an mRNA splice variant of VEGF. Alternative exon usage results in a 165 amino acid product that is identical to VEGF₁₆₅ except for 6 unique amino acids at the C-terminus. VEGF₁₆₅b inhibits proliferation and migration of endothelial cells and is down-regulated in renal cell carcinomas.

Item	Value
Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names	MVCD1, VAS, VEGF, VEGF-A, VEGFA, VEGFMGC70609, VPF, VPFvascular endothelial growth factor, Vascular permeability factor, Vasculotropin, vascular endothelial growth factor A

Citations for Human VEGF_165b Antibody (10)

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Citations are publications that use Bio-Techne products. Selected citations for Human VEGF_165b Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

T Jia et al. (2021), FGF-2 promotes angiogenesis through a SRSF1/SRSF3/SRPK1-dependent axis that controls VEGFR1 splicing in endothelial cells Bmc Biology, 19(1):173.
PMID: 34433435
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

AF Stassi et al. (2019), Contribution of the VEGF system to the follicular persistence associated with bovine cystic ovaries Theriogenology, 138(0):52-65.
PMID: 31301448
Species: Bovine
Sample Types: Tissue Homogenates
Applications: Western Blot

AF Stassi et al. (2019), Contribution of the VEGF system to the follicular persistence associated with bovine cystic ovaries Theriogenology, 138(0):52-65.
PMID: 31301448
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Van't Hull E et al. (2014), Bone marrow-derived cells are implicated as a source of lymphatic endothelial progenitors in human breast cancer. Oncoimmunology, 3(0):e29080.
PMID: 25101222
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Bills V et al. (2014), The role of VEGF-A165b in trophoblast survival. BMC Pregnancy Childbirth, 14(0):278.
PMID: 25128406
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Bates et al. (2013), Detection of VEGF-A(xxx)b isoforms in human tissues. PLoS ONE, 8(7):e68399.
PMID: 23935865
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

Merdzhanova G et al. (2010), The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo. Oncogene, 29(39):5392-403.
PMID: 20639906
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Rennel ES et al. (2009), VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo. Br. J. Cancer, 101(7):1183-93.
PMID: 19707198
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development

Bates DO et al. (2006), The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term. Clin. Sci., 110(5):575-85.
PMID: 16451124
Species: Human
Sample Types: BALF
Applications: ELISA Development

Varet et al. (0), VEGF in the lung: a role for novel isoforms. Am J Physiol Lung Cell Mol Physio298(6):L768-74.
PMID: 20228180

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Customer Reviews for Human VEGF_165b Antibody (2)

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Human VEGF165b Antibody

Name: Anonymous

Application: IHC

Sample Tested: Ovarian cyst

Species: Human

Verified Customer | Posted 02/15/2022
Human VEGF165b Antibody

Name: Anonymous

Application: IHC

Sample Tested: Placental tissue

Species: Human

Verified Customer | Posted 10/03/2021

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Product Documents for Human VEGF_165b Antibody

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Human VEGF_165b Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human VEGF_165b Antibody

Scientific Data Examples for Human VEGF_165b Antibody

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: VEGF

Citations for Human VEGF_165b Antibody (10)

Customer Reviews for Human VEGF_165b Antibody (2)

Customer Images

Human VEGF165b Antibody

Human VEGF165b Antibody

Product Documents for Human VEGF_165b Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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Human VEGF165b Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human VEGF165b Antibody

Scientific Data Examples for Human VEGF165b Antibody

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: VEGF

Citations for Human VEGF165b Antibody (10)

Customer Reviews for Human VEGF165b Antibody (2)

Customer Images

Human VEGF165b Antibody

Human VEGF165b Antibody

Product Documents for Human VEGF165b Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Human VEGF_165b Antibody

Applications for Human VEGF_165b Antibody

Scientific Data Examples for Human VEGF_165b Antibody

Citations for Human VEGF_165b Antibody (10)

Customer Reviews for Human VEGF_165b Antibody (2)

Product Documents for Human VEGF_165b Antibody