Human VAMP-5 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7366AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Specificity
Detects human VAMP-5 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) VAMP-7 is observed, and less than 1% cross-reactivity with rhVAMP-1, rhVAMP-2, and rhVAMP-8 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human VAMP-5 Alexa Fluor™ Plus 488-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Background: VAMP-5
VAMP-5 (vesicle associated membrane protein 5; also myobrevin) is a member of the synaptobrevin family of proteins. Although its predicted MW is 11 kDa, it runs anomalously at 16 kDa in SDS-Page. VAMP-5 is a type IV transmembrane (TM) protein (i.e.-a type II TM protein whose C-terminus is almost completely transmembrane) that is found in both trans-Golgi and plasma membranes of myotubes (mature skeletal and cardiac muscle cells). While VAMPs are commonly associated with vesicle fusion to t-SNARE in cell membranes, VAMP-5 appears to show no such activity. Human VAMP-5 is 116 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-72) with one vSNARE coiled-coil homology domain, a 21 aa TM segment, and 23 aa C-terminal luminal domain. Over aa 1-72, human VAMP-5 shares 75% aa identity with mouse VAMP-5.
Long Name
Vesicle-Associated Membrane Protein 5
Alternate Names
Myobrevin, VAMP5
Gene Symbol
VAMP5
UniProt
Additional VAMP-5 Products
Product Specific Notices for Human VAMP-5 Alexa Fluor™ Plus 488-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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