Detects human Trypsin 1/PRSS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Trypsin 2 and rhTrypsin 3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Trypsin 1/PRSS1 Antibody

Trypsin 1/PRSS1 in Human Pancreas.

Trypsin 1/PRSS1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Trypsin 1/PRSS1 Monoclonal Antibody (Catalog # MAB3848) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Trypsin 1/PRSS1 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Trypsin 1/PRSS1 (Catalog # 3848-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Trypsin 1/PRSS1 (Catalog # 3848-SE)

Reviewed Applications

Read 2 reviews rated 5 using AF3848 in the following applications:

Immunohistochemistry-Frozen (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin 1/PRSS1

Human Trypsin 1, encoded by the PRSS1 gene, is also known as Cationic Trypsinogen (1). Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes (2). Mutations in the PRSS1 gene can cause hereditary pancreatitis (3).

References

Emi, M. et al. (1986) Gene 41:305.
Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1483, Academic Press, San Diego.
Teich, N. et al. (2006) Hum. Mutat. 27:721.

Alternate Names

PRSS1, PTRYI, TRY4, Trygn16, Trypsinogen 1

Entrez Gene IDs

5644 (Human); 114228 (Mouse); 24691 (Rat)

Gene Symbol

PRSS1

UniProt

P07477

Additional Trypsin 1/PRSS1 Products

Product Documents for Human Trypsin 1/PRSS1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Trypsin 1/PRSS1 Antibody

For research use only

Citations
Reviews

Human Trypsin 1/PRSS1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3848

Key Product Details

Species Reactivity

Validated:

Cited:

Applications