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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgM Clone # TRA-1-81

Product Specifications

Immunogen

Human embryonal carcinoma cell line 2102Ep

Specificity

Detects human TRA-1-81 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human TRA-1-81 PE-conjugated Antibody

Detection of TRA-1-81 antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of TRA-1-81 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-81 PE-conjugated Monoclonal Antibody (Catalog # FAB8495P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of TRA-1-81 in iPSC cells by Flow Cytometry.

iPSC cells were stained with Mouse Anti-Human TRA-1-81 PE-conjugated Monoclonal Antibody (Catalog # FAB8495P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRA-1-81 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: BG01V human embryonic stem cells, iPSC cells

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-81

The TRA-1-81 antibody reacts with a high-molecular-mass carbohydrate epitope on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells.  TRA-1-81 is an epitope expressed on podocalyxin, also known as Podocalyxin-Like Protein-1 (PCLP1 or PODXL), a type I transmembrane glycoprotein.

Long Name

Tumor-related Antigen-1-81

Alternate Names

TRA181

Entrez Gene IDs

5420 (Human)

Gene Symbol

PODXL

Additional TRA-1-81 Products

Product Documents for Human TRA-1-81 PE-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TRA-1-81 PE-conjugated Antibody

For research use only

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