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Human TLR1 APC-conjugated Antibody

Bio-Techne includes R&D Systems | Catalog # FAB1484A

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FAB1484A

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG

Product Summary for Human TLR1 APC-conjugated Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TLR1
Ser22-Asn578
Accession # AAC34137

Specificity

Detects human TLR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) TLR1 is observed and less than 5% cross-reactivity with recombinant human (rh) TLR3, rhTLR4, and rmTLR6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TLR1 APC-conjugated Antibody

Detection of TLR1 antibody in Human Monocytes antibody by Flow Cytometry.

Detection of TLR1 in Human Monocytes by Flow Cytometry.

Human monocytes were stained with Goat Anti-Human TLR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1484A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TLR1 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human monocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using FAB1484A in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TLR1

The Toll-like family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2). TLRs contain a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL-1 receptor (TIR) domain. Mature human TLR1 consists of a 556 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (3, 4). Within the ECD, human TLR1 shares 63% aa sequence identity with human TLR6 and 20%‑43% aa sequence identitity with human TLR2, -3, -4, -5, -7, -8, -9, and -10. It shares 73% and 71% aa sequence identity with mouse and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand-independent association with TLR2 (5‑8). TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands (9‑11). TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols (6, 10‑12). Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization and activation of NF kappaB (7, 11, 13).

References

  1. Miyake, K. (2007) Semin. Immunol. 19:3.
  2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  3. Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
  4. Matsushima, N. et al. (2007) BMC Genomics 8:124.
  5. Ochoa, M.-T. et al. (2003) Immunology 108:10.
  6. Takeuchi, O. et al. (2002) J. Immunol. 169:10.
  7. Triantafilou, M. et al. (2006) J. Biol. Chem. 281:31002.
  8. Sandor, F. et al. (2003) J. Cell Biol. 162:1099.
  9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
  10. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. 97:13766.
  11. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
  12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
  13. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.

Long Name

Toll-like Receptor 1

Alternate Names

CD281

Entrez Gene IDs

7096 (Human); 21897 (Mouse); 305354 (Rat)

Gene Symbol

TLR1

UniProt

Additional TLR1 Products

Product Documents for Human TLR1 APC-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TLR1 APC-conjugated Antibody

For research use only

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