Human SUMO Pan Specific Antibody

R&D Systems, part of Bio-Techne | Catalog # A-714

R&D Systems, part of Bio-Techne
Discontinued Product
A-714 has been discontinued. View all SUMO products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 852721

View all SUMO Antibodies »

Product Specifications

Immunogen

Purified, recombinant human SUMO2
Accession # P61956

Specificity

This antibody detects endogenous, human SUMOylated proteins in Western blots, and has similar sensitivity when tested against purified, recombinant SUMO1, SUMO2, SUMO3 and SUMO4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human SUMO Pan Specific Antibody

Detection of Human SUMO antibody by Western Blot.

Detection of Human SUMO by Western Blot.

Western blot shows lysates of pan-SUMO. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO Pan Specific Monoclonal Antibody (Catalog # A-714) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SUMO at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SUMO Pan Specific Antibody

Application
Recommended Usage

Western Blot

0.1-0.5 µg/mL
Sample: Human Pan-SUMO

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: SUMO

Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation. Unlike ubiquitination which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, SUMO2, SUMO3, and SUMO4 are all translated as propeptides, containing C-terminal prosegments following the diglycine motif that marks the end of the mature forms. Following prosegment cleavage, SUMO1, 2, and 3 may then be enzymatically attached to a lysine on a target protein. It is not clear whether SUMO4 is processed in a similar fashion.

Long Name

Small Ubiquitin-like Modifiers

Alternate Names

DAP1;GMP1;OFC10;PIC1;SENP2;Small ubiquitin-related modifier;Small ubiquitin-related modifier 1;SMT3;SMT3C;SMT3H3;SUMO1;UBL1

UniProt

P61956

Product Documents for Human SUMO Pan Specific Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SUMO Pan Specific Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions

For research use only

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