Human SPRY4 Alexa Fluor™ Plus 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5070AFP594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SPRY4
Specificity
Detects endogenous human SPRY4 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human SPRY4 Alexa Fluor™ Plus 594-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: SPRY4
SPRY4 (sprouty 4) is a member of the sprouty family of proteins. It blocks tyrosine kinase growth factor activation of the MAPK signaling pathway, possibly through an interaction with either Raf1 or TESK1. Human SPRY4 is 322 amino acids (aa) in length. It contains three SH3-binding domains (aa 3‑9; 25‑31; 168‑174), an NLS (aa 87‑93), a PEST sequence (aa 118‑133), and a Cys-rich/Zn++-finger domain that binds both TESK1 and Raf1 (aa 182‑298). There are two variant isoforms. One is a short form that shows an alternate start site at Met24, while a second shows a 10 aa substitution for aa 120‑129, followed by a premature truncation. Over aa 1‑177, human SPRY4 is 89% aa identical to canine SPRY4. Mouse SPRY4 is orthologous to the human short form, and shares 90% aa identity with human SPRY4.
Long Name
Sprouty Homolog 4
Alternate Names
protein sprouty homolog 4, sprouty homolog 4 (Drosophila), Spry-4
Gene Symbol
SPRY4
UniProt
Additional SPRY4 Products
Product Specific Notices for Human SPRY4 Alexa Fluor™ Plus 594-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
Loading...
Loading...
Loading...
Loading...