Human SPRY1 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6097AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 669534
Product Specifications
Immunogen
E.coli-derived recombinant human SPRY1
Specificity
Detects human SPRY1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SPRY2, 3, or 4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human SPRY1 Alexa Fluor™ Plus 405-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: SPRY1
SPRY1 (sprouty homolog 1) is a 34-38 kDa member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-gamma 1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319 amino acids (aa) in length. It contains one CBL-TKB binding site (aa 51-57) that is phosphorylated at Tyr53, a Ser-rich region (aa 112-131), and a Cys-rich domain (aa 181-306) that mediates intracellular translocation. SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes. Over aa 1-178, human SPRY1 shares 76% aa identity with mouse SPRY1.
Long Name
Sprouty Homolog 1
Alternate Names
Drosophila, homolog of, 1 (antagonist of FGF signaling), sprouty homolog 1, antagonist of FGF signaling (Drosophila), spry-1
Gene Symbol
SPRY1
UniProt
Additional SPRY1 Products
Product Specific Notices for Human SPRY1 Alexa Fluor™ Plus 405-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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