Human SOS2 Alexa Fluor™ Plus 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6260AFP647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SOS2
Specificity
Detects human SOS2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human SOS2 Alexa Fluor™ Plus 647-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: SOS2
SOS2 (Son of sevenless homolog 2) is a 160‑170 kDa protein that regulates Ras signaling. It is widely expressed, and serves as a mediator of guanine nucleotide phosphate exchange. In the resting cell, cytoplasmic SOS2 forms a heterodimer with Grb2, and a heterotrimer with Eps8 and E3b1. Upon RTK activation, the SOS heterodimer is recruited to the cell membrane, where it contacts GDP‑bound Ras. This allows it to facilitate a GTP‑for‑GDP exchange that activates Ras. The heterotrimer interacts with Rac on actin filaments. SOS2 activity parallels that of SOS1. Relative to SOS1, however, SOS2 binds Grb2 with higher affinity and shows less biological activity due to a shorter half‑life. Human SOS2 is 1332 amino acids (aa) in length and contains one histone fold (aa 97‑169), PH (aa 439‑546) and REM (aa 595‑739) domains that interact with Ras, and a Pro‑rich region that binds to Grb2 (aa 1126‑1242).
Long Name
Son of Sevenless Homolog 2
Alternate Names
FLJ25596, guanine nucleotide exchange factor, guanine nucleotide releasing factor, son of sevenless (Drosophilia) homolog 2, son of sevenless homolog 2, son of sevenless homolog 2 (Drosophila), SOS-2
Gene Symbol
SOS2
UniProt
Additional SOS2 Products
Product Specific Notices for Human SOS2 Alexa Fluor™ Plus 647-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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