Sirtuin 3 (SIRT3; SIR2-like protein 3) is a 28-30 kDa, NAD⁺-dependent class III member of the sirtuin protein family. It is widely expressed and found apparently in both the nucleus where it deacetylates histones, and in the mitochondrial matrix where it is involved in the regulation of both oxidative stress and the physiology associated with fasting or caloric restriction. Relative to the mitochondria, SIRT3 deacetylates multiple enzyme substrates including SOD2, IDH2, LCAD, OTC and HMGCS2, an action that results in enzyme activation. Human SIRT3 is 399 amino acids (aa) in length. It contains a mitochondrial targeting sequence within the first 100 aa, followed by a sirtuin-type deacetylase domain (aa 126-382) that contains an NAD binding segment (aa 145-165). SIRT3 is initially synthesized as a 42-44 kDa "precursor" in the cytosol. From here, it can translocate into mitochondria where it is cleaved between Arg99Arg100 to generate a 28-30 kDa short form. There is one potential alternative start site at Met143. Over aa 101-399, human and mouse SIRT3 share 84% aa sequence identity.

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Entrez Gene IDs

23410 (Human); 64384 (Mouse); 293615 (Rat)

Gene Symbol

SIRT3

UniProt

Q9NTG7 (Human)

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Product Specific Notices for Human Sirtuin 3/SIRT3 Alexa Fluor® 350-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human Sirtuin 3/SIRT3 Alexa Fluor® 350-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7488U

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Background: Sirtuin 3/SIRT3