Detects human Sirtuin 2/SIRT2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SIRT1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human Sirtuin 2/SIRT2 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sirtuin 2/SIRT2

Sirtuin 2, encoded by the SIRT2 gene, is also known as SIR2 (silent information regulator 2)-like protein 2. It is a nicotinamide adenine dinucleotide (NAD)-dependent histone/protein deacetylase (1, 2). The SIR2 family of enzymes is classified as class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging (3, 4). Unlike class I and class II HDACs, the enzymatic activity of class III HDACs is NAD dependent and insensitive to HDAC inhibitor trichostatin A (5).

References

Frye, R.A. (1999) Biochem. Biophys. Res. Commun. 260:273.
North, B.J. et al. (2003) Mol. Cell. 11:437.
North, B.J. and Verdin, E. (2004) Genome Biol. 5:224.
Marmorstein, R. (2004) Biochem. Soc. Trans. 32:904.
Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262:75.

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Entrez Gene IDs

22933 (Human); 64383 (Mouse); 361532 (Rat)

Gene Symbol

SIRT2

UniProt

Q8IXJ6

Additional Sirtuin 2/SIRT2 Products

Product Documents for Human Sirtuin 2/SIRT2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Sirtuin 2/SIRT2 Antibody

For research use only

Citations
Reviews

Human Sirtuin 2/SIRT2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4358

Key Product Details

Species Reactivity

Validated:

Cited:

Applications