Sigma-1 R was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Sigma-1 R Monoclonal Antibody (Catalog # MAB1076) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Sigma-1 R Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sigma-1 R

Sigma non-opioid intracellular receptor 1 (SIGMAR1, OPRS1 or Sigma1 receptor) is a 223 aminoacids (aa), 25KDa chaperone protein at the endoplasmic reticulum (ER) that modulates calcium signaling through the IP3 receptor. Sigmar1 functions as a lipid transporter and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. In Human, at least 4 other isoforms have been found, ranging from 12-22 KDa.

Long Name

Sigma Non-opioid Intracellular Receptor 1

Alternate Names

ALS16, OPRS1, SIG-1R, Sigma-1R, Sigma1R, SIGMAR1, SRBP

Entrez Gene IDs

10280 (Human); 18391 (Mouse); 29336 (Rat)

Gene Symbol

SIGMAR1

UniProt

Q99720

Additional Sigma-1 R Products

Product Documents for Human Sigma-1 R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Sigma-1 R Antibody

For research use only

Citations
Reviews

Human Sigma-1 R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1076

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Sigma-1 R Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Sigma-1 R Antibody

Sigma-1 R in HeLa Human Cell Line.