Human SF20/MYDGF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1147
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SF20/MYDGF
Val32-Leu173
Accession # Q969H8
Val32-Leu173
Accession # Q969H8
Specificity
Detects human SF20/MYDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SF20/MYDGF is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SF20/MYDGF Antibody
Detection of Human SF20/MYDGF by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Antibody (Catalog # AF1147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SF20/MYDGF at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SF20/MYDGF by Simple WesternTM.
Simple Western lane view shows lysates of DU145 human prostate carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SF20/MYDGF at approximately 22 kDa (as indicated) using 25 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1147) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of SF20/MYDGF by Western Blot
rhMYDGF promotes HCAEC proliferation. HCAECs were stimulated with rhMYDGF (100 ng/mL unless otherwise indicated) in the absence or presence of PD98059 (PD, 20 µmol/L) or Static (1 nmol/L), as indicated. A, Representative immunoblots showing cyclin D1 and GAPDH expression in HCAECs. B, Representative immunoblots showing cyclin D1 and GAPDH expression in HCAECs stimulated for 12 h. C‐D, Western blot analysis was performed to examine the protein levels of cyclin D1. *P < .05, **P < .01 and ***P < .001 vs baseline (two independent‐sample t test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31758636), licensed under a CC-BY license. Not internally tested by R&D Systems.
Applications for Human SF20/MYDGF Antibody
Application
Recommended Usage
Simple Western
25 µg/mL
Sample: DU145 human prostate carcinoma cell line
Sample: DU145 human prostate carcinoma cell line
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line
Sample: Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SF20/MYDGF
Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted) bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.
Long Name
Stromal Cell-derived Growth Factor SF20
Alternate Names
C19orf10, Ly6elg, MYDGF
Gene Symbol
MYDGF
UniProt
Additional SF20/MYDGF Products
Product Documents for Human SF20/MYDGF Antibody
Product Specific Notices for Human SF20/MYDGF Antibody
For research use only
Loading...
Loading...
Loading...
Loading...