Detects human Serpin C1/Antithrombin-III in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse (rm) Serpin C1 is observed and less than 2% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin F2, rmSerpin D1 and rhPEDF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human Serpin C1/Antithrombin-III Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin C1/
Antithrombin-III (Catalog # 1267-PI), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serpin C1/Antithrombin-III (Catalog # 1267-PI)

Neutralization

Measured by its ability to neutralize Recombinant Human Serpin C1 (0.8 µg/mL, Catalog # 1267-PI) inhibition of Recombinant Human Coagulation Factor II/Thrombin (0.05 µg/mL, Catalog # 1473-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND₅₀) is typically 4.6 µg/mL.

Reviewed Applications

Read 1 review rated 5 using AF1267 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin C1/Antithrombin-III

Serpin C1 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa (2). Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases (3). For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery (4).

References

Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Chuang, Y.-J. et al. (2001) Biochemistry 40:6670.
Vinazzer, H. (1999) Semin. Thromb. Hemost. 25:257.
Risberg, B. (1998) Blood Coagul. Fibrinolysis Suppl. 3:S3.

Alternate Names

Antithrombin-III, ATIII

Entrez Gene IDs

462 (Human); 11905 (Mouse)

Gene Symbol

SERPINC1

UniProt

P01008

Additional Serpin C1/Antithrombin-III Products

Product Documents for Human Serpin C1/Antithrombin-III Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Serpin C1/Antithrombin-III Antibody

For research use only

Citations
Reviews

Human Serpin C1/Antithrombin-III Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1267

Key Product Details

Species Reactivity

Validated:

Cited:

Applications