SCGF/CLEC11a was detected in immersion fixed paraffin-embedded sections of Lung Cancer using Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB19041) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human SCGF/CLEC11a Antibody

Application

Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Lung Cancer.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCGF/CLEC11a

Stem Cell Growth Factor (SCGF) is a secreted sulfated glycoprotein that belongs to the C-type lectin superfamily (CLEC11a) and is expressed primarily in hematopoietic tissues. It functions as a growth factor for primitive hematopoietic progenitor cells. SCGF-beta is a truncated variant of SCGF-alpha that has aa 78 amino acid deletion and lacks a functional calcium-dependent carbohydrate-recognition domain.

Long Name

Stem Cell Growth Factor/C-type Lectin Domain Family 11, Member A

Alternate Names

CLEC11a, CLECSF3, LSLCL

Entrez Gene IDs

6320 (Human); 20256 (Mouse); 29313 (Rat)

Gene Symbol

CLEC11A

UniProt

BAA21499

Additional SCGF/CLEC11a Products

Product Documents for Human SCGF/CLEC11a Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SCGF/CLEC11a Antibody

For research use only

Citations
Reviews

Human SCGF/CLEC11a Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB19041

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SCGF/CLEC11a Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SCGF/CLEC11a Antibody

Detection of SCGF/CLEC11a in Lung Cancer.