Human SCF/c-kit Ligand Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB655
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Western Blot, Neutralization, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 13302
Product Specifications
Immunogen
E. coli-derived recombinant human SCF
Glu26-Ala189
Accession # P21583
Glu26-Ala189
Accession # P21583
Specificity
Detects recombinant human SCF in Western blots. In sandwich ELISAs, no significant cross-reactivity or interference was observed with 36 other cytokines.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human SCF/c-kit Ligand Antibody
Human SCF / c-kit Ligand ELISA Standard Curve
Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c-kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human SCF/c-kit Ligand Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC)
Sample: Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC)
Human SCF/c-kit Ligand Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCF/c-kit Ligand
SCF, also known as c-kit ligand, mast cell growth factor (MGF) and steel-factor (SLF), is a pleiotropic cytokine that plays essential roles in gametogenesis, melanogenesis and early stages of hematopoiesis. SCF can exist either as a membrane bound form or as a soluble form consisting of the first 164 or 165 amino acids of the extracellular domain.
Long Name
Stem Cell Factor
Alternate Names
c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF
Gene Symbol
KITLG
UniProt
Additional SCF/c-kit Ligand Products
Product Documents for Human SCF/c-kit Ligand Antibody
Product Specific Notices for Human SCF/c-kit Ligand Antibody
For research use only
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