SALM2 was detected in immersion fixed paraffin-embedded sections of human cerebellum using Human SALM2/LRFN1 Monoclonal Antibody (Catalog # MAB5669) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SALM2/LRFN1 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cerebellum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALM2/LRFN1

SALM2 (synaptic adhesion-like molecule 2), also called LRFN1 (leucine-rich repeat and fibronectin type-III domain-containing protein 1) is a 105 kDa member of the LRFN family of type I transmembrane glycoproteins. The 740 amino acid (aa) mature human SALM2 contains a 505 aa extracellular domain that includes seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain. Mature human SALM2 shares 99% aa identity with mouse and rat SALM2. SALM2 co-localizes with both pre- and post-synaptic proteins at excitatory synapses in mature neurons and promotes neurite outgrowth.

Long Name

Synaptic Adhesion-like Molecule 2/Leucine-rich Repeat and Fibronectin Type III Domain Containing 1

Alternate Names

LRFN1

Entrez Gene IDs

57622 (Human); 365222 (Rat)

Gene Symbol

LRFN1

UniProt

Q9P244

Additional SALM2/LRFN1 Products

Product Documents for Human SALM2/LRFN1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SALM2/LRFN1 Antibody

For research use only

Citations
Reviews

Human SALM2/LRFN1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5669

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SALM2/LRFN1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SALM2/LRFN1 Antibody

SALM2 in Human Cerebellum.