Human S100A8/S100A9 Heterodimer Antibody

Name: Human S100A8/S100A9 Heterodimer Antibody
Brand: R&D Systems
SKU: MAB45701
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB45701

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Recombinant Monoclonal Antibody.

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human S100A8/S100A9 heterodimer Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9) Accession # P05109 (S100A8) and P06702 (S100A9)
Applications:	Dual RNAscope ISH-IHC, Immunocytochemistry/Immunofluorescence, Immunohistochemistry
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 1099F
Specificity:	Detects human S100A8/S100A9 in direct ELISAs. In ELISAs, it detects recombinant human S100A8/S100A9 heterodimer but it does not detect recombinant human S100A8 or S100A9 monomers.

View Full Technical Details for Human S100A8/S100A9 Heterodimer Antibody

Applications for Human S100A8/S100A9 Heterodimer Antibody

	Recommended Concentration	Sample
Immunohistochemistry	10-25 µg/mL	See below
Dual RNAscope ISH-IHC	5-25 µg/mL	See below
Immunocytochemistry	3-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human S100A8/S100A9 Heterodimer Antibody

	Immunocytochemistry S100A8/S100A9 Heterodimer in HL60 Human Cell Line. S100A8/S100A9 Heterodimer was detected in immersion fixed HL60 human promyelocytic leukemia cell line using Rabbit Anti-Human S100A8/S100A9 Heterodimer Monoclonal Antibody (Catalog # MAB45701) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm (punctate). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Immunohistochemistry S100A8/S100A9 Heterodimer in Human Lung Cancer Tissue. S100A8/S100A9 Heterodimer was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Rabbit Anti-Human S100A8/S100A9 Heterodimer Monoclonal Antibody (Catalog # MAB45701) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tumor cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Dual RNAscope ISH-IHC Compatible S100A8/S100A9 in Human Metastatic Tonsil Cancer Tissue Using Dual RNAscope^®ISH and IHC. Syndecan-1/CD138 mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil cancer tissue probed with ACD RNAScope^®Probe (Catalog # 425271) and stained using ACD RNAscope^®2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Rabbit Anti-Human S100A8/S100A9 Monoclonal Antibody (Catalog# MAB45701) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC003) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: S100A8/S100A9 Heterodimer

S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (3-5). The 10 kDa human S100A8 and 14 kDa S100A9 each contain two EF‑hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (6, 7). In the presence of calcium or zinc, S100A8 and S100A9 associate into non-covalent homodimers and 34-35 kDa heterodimers with each other (8-10). The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (11). The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation (3, 12). S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (4, 5, 9).

Item	Value
References	Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250. Odink, K. et al. (1987) Nature 330:80. Dorin, J.R. et al. (1987) Nature 326:614. Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462. Ryckman, C. et al. (2003) J. Immunol. 170:3233. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298. Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841. Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948.
Long Name	S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9
Entrez Gene IDs	6279 (Human)
Alternate Names	Calprotectin, S100A8/S100A9 Heterodimer

Product Specific Notices for Human S100A8/S100A9 Heterodimer Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

Citations for Human S100A8/S100A9 Heterodimer Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human S100A8/S100A9 Heterodimer Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

F Real et al. (2022), S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo Nature Communications, 13(1):5956.
PMID: 36220814

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Human S100A8/S100A9 Heterodimer Antibody

Name: Anonymous

Application: IHC

Sample Tested: Brain tissue

Species: Human

Verified Customer | Posted 10/23/2022

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Human S100A8/S100A9 Heterodimer Antibody

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Applications for Human S100A8/S100A9 Heterodimer Antibody

Scientific Data Examples for Human S100A8/S100A9 Heterodimer Antibody

Immunocytochemistry

Immunohistochemistry

Dual RNAscope ISH-IHC Compatible

Formulation, Preparation and Storage

Reconstitution Calculator

Background: S100A8/S100A9 Heterodimer

Product Specific Notices for Human S100A8/S100A9 Heterodimer Antibody

Citations for Human S100A8/S100A9 Heterodimer Antibody (1)

Customer Reviews for Human S100A8/S100A9 Heterodimer Antibody (1)

Customer Images

Human S100A8/S100A9 Heterodimer Antibody

Product Documents for Human S100A8/S100A9 Heterodimer Antibody

Product Datasheets

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