Human Ryk Alexa Fluor® 647-conjugated Antibody

Name: Human Ryk Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems
SKU: AF4907R

R&D Systems, part of Bio-Techne | Catalog # AF4907R

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Product Datasheet / COA / SDS

Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Ryk Ala47-Thr224 Accession # NP_001005861
Applications:	CyTOF-ready, Flow Cytometry, Western Blot
Label:	Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)
Source:	Polyclonal Sheep IgG
Specificity:	Detects human Ryk in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant mouse Ryk is observed.

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Technical Data
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Applications for Human Ryk Alexa Fluor® 647-conjugated Antibody

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Flow Cytometry	Optimal dilution of this antibody should be experimentally determined.
CyTOF-ready	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ryk

RYK (related to tyrosine (Y) kinase; also H-RYK) is a 70-90 kDa, atypical member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt, and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Human RYK is 604 amino acids (aa) in length (SwissProt Accession # P34925). It contains a 25 aa signal sequence and a 199 aa extracellular region (aa 26-224) that shows one WIF-1-like domain (aa 63‑191). The cytoplasmic region possesses a nonfunctional Ser/Thr protein kinase domain (aa 327-600). One isoform exists that shows a 31 aa substitution between aa’s 18-46. Over aa 47-224, human RYK is 94% aa identical to mouse RYK.

Item	Value
Long Name	Receptor Type Tyrosine Protein Kinase RYK
Entrez Gene IDs	6259 (Human); 20187 (Mouse); 140585 (Rat)
Alternate Names	D3S3195, EC 2.7.10, EC 2.7.10.1, JTK5, JTK5A, JTK5A protein tyrosine kinase, RYK receptor-like tyrosine kinase, RYK1, Ryk, hydroxyaryl-protein kinase, tyrosine-protein kinase RYK

Product Specific Notices for Human Ryk Alexa Fluor® 647-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human Ryk Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4907R

Key Product Details

Applications for Human Ryk Alexa Fluor® 647-conjugated Antibody

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: Ryk

Product Specific Notices for Human Ryk Alexa Fluor® 647-conjugated Antibody

Customer Reviews for Human Ryk Alexa Fluor® 647-conjugated Antibody

Product Documents for Human Ryk Alexa Fluor® 647-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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