Western Blot shows lysates of Daudi human Burkitt's lymphoma cell line, SUP-T1 human T cell lymphoblastic lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human ROR gamma/RORC/NR1F3 Monoclonal Antibody (Catalog # MAB11629) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ROR gamma/RORC/NR1F3 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human ROR gamma/RORC/NR1F3 Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, SUP-T1 human T cell lymphoblastic lymphoma cell line and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR gamma/RORC/NR1F3

ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑121 human ROR gamma shares 97% identity with mouse ROR gamma.

Long Name

Retinoic Acid-Related Orphan Receptor gamma

Alternate Names

NR1F3, RORC, RZRG

Entrez Gene IDs

6097 (Human)

Gene Symbol

RORC

UniProt

P51449

Additional ROR gamma/RORC/NR1F3 Products

Product Documents for Human ROR gamma/RORC/NR1F3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ROR gamma/RORC/NR1F3 Antibody

For research use only

Citations
Reviews

Human ROR gamma/RORC/NR1F3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11629

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ROR gamma/RORC/NR1F3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ROR gamma/RORC/NR1F3 Antibody

Detection of Human ROR gamma/RORC/NR1F3 by Western Blot.