HUVEC cells were stained with Goat Anti-Human ROBO4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2366, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ROBO4 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human ROBO4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO4

ROBO4 belongs to the ROBO family, which are molecular guidance receptors that interact with Slit ligand to regulate axon guidance, neuronal migration and leukocyte chemotaxis. The expression of ROBO4 is restricted in the endothelium, notably in areas of angiogenesis.

Long Name

Roundabout Homolog 4

Alternate Names

ECSM4, FLJ20798, Magic roundabout, MGC133352, MGC133353, MRB, roundabout homolog 4, roundabout homolog 4, magic roundabout (Drosophila)

Entrez Gene IDs

54538 (Human); 74144 (Mouse)

Gene Symbol

ROBO4

UniProt

Q8WZ75

Additional ROBO4 Products

Product Documents for Human ROBO4 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ROBO4 Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ROBO4 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2366

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all ROBO4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ROBO4 Biotinylated Antibody

Detection of ROBO4 in HUVEC cells by Flow Cytometry