Human Rex-1/ZFP42 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF3598
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Rex‑1/ZFP42
Ser2-Lys150
Accession # NP_777560
Ser2-Lys150
Accession # NP_777560
Specificity
Detects human Rex‑1/ZFP42 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Rex-1/ZFP42 Biotinylated Antibody
Rex-1 in BG01V Human Stem Cells.
Rex-1 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Human Rex-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Rex-1/ZFP42 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human Rex-1
Sample: Recombinant Human Rex-1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rex-1/ZFP42
Rex-1, also named zinc finger protein 42 (Zfp-42), is a 32 kDa transcription factor belonging to the kruppel C2H2-type zinc-finger protein family. It is a putative stem cell marker and is highly expressed in embryonic and adult stem cells. Over the region used for immunization, human and mouse Rex-1 share 47% amino acid sequence identity.
Long Name
Zinc Finger Protein 42
Alternate Names
Rex1, ZFP42
Gene Symbol
ZFP42
UniProt
Additional Rex-1/ZFP42 Products
Product Documents for Human Rex-1/ZFP42 Biotinylated Antibody
Product Specific Notices for Human Rex-1/ZFP42 Biotinylated Antibody
For research use only
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