Human Reg1A/B Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4937V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Reg1A
Gln23-Asn166
Accession # P05451
Gln23-Asn166
Accession # P05451
Specificity
Detects human Reg1A in direct ELISAs. In direct ELISAs, approximately 80% cross-reactivity with recombinant human (rh) Reg1B is observed, approximately 20% cross-reactivity with recombinant mouse Reg1, and less than 5% cross-reactivity with rhReg3A and re
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Reg1A/B Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Reg1A
Reg1A (Regenerating gene IA/ alpha; also REG, ICRF, PSPS, PTP and Lithostathine-1 alpha) is a secreted, variably glycosylated 15-22 kDa type I member of the REG gene family of proteins. It is induced in proliferating cell types such as colonic epithelium, islet beta-cells, and multiple tumor types, and is constitutively expressed by pancreatic acinar cells. Thus, Reg1A is found both in blood (ng/mL quantities) and pancreatic secretions. It would appear that Reg1A plays a role in blocking apoptosis, and is upregulated by both IL-22 and IL-6. Human Reg1A is synthesized as a 166 amino acid (aa) precursor. It contains a 22 aa signal sequence plus a 144 aa mature region that is characterized by the presence of three intrachain disulfide bonds, one C-type lectin domain (aa 34-164), and a utilized N-terminal O-linked glycosylation site (Thr27). Variablility in the glycosylation pattern, plus proteolytic cleavage after Arg11 generates Reg1A isoforms in the 15-22 kDa range. There is one potential isoform variant that shows an alternative start site at Met9. Mature (aa 23-166) human Reg1A shares 76% and 69% aa sequence identity with mouse and rat Reg1A, respectively.
Reg1B, also known as secretory pancreatic stone protein 2 and lithostathine 1 beta, is a type I subclass member of the Reg family, which comprises secreted proteins with a C-type lectin domain. Reg1B is highly related to Reg1A, a protein that is implicated in islet cell regeneration and diabetogenesis.Long Name
Regenerating Islet-derived 1A
Alternate Names
ICRF, PSP, PTP
Entrez Gene IDs
5967 (Human)
Gene Symbol
REG1A
UniProt
Additional Reg1A Products
Product Specific Notices for Human Reg1A/B Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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