Human RBFOX3/NeuN MAb (Clone 1094645)

Detection of RBFOX3 in Human Cerebellum via seqIF™ staining on COMET™​

RBFOX3 was detected in immersion fixed paraffin-embedded sections of human Cerebellum using Mouse Anti-Human RBFOX3, Monoclonal Antibody (Catalog #MAB11665) at 25ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the neuronal nuclei. Protocol available in COMET™ Panel Builder.

Detection of RBFOX3/NeuN in Human Cerebellum.

RBFOX3/NeuN was detected in immersion fixed paraffin-embedded sections of human cerebellum using Mouse Anti-Human RBFOX3/NeuN Monoclonal Antibody (Catalog # MAB11665) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human RBFOX3/NeuN by Western Blot.

Western Blot shows lysates of human hippocampus tissue. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human RBFOX3/NeuN Monoclonal Antibody (Catalog # MAB11665) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for RBFOX3/NeuN at approximately 40 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.