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Human RANK/TNFRSF11A Antibody

Bio-Techne includes R&D Systems | Catalog # AF683

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AF683-SP
AF683

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Agonist Activity, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human RANK/TNFRSF11A Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human RANK
Gln29-Gly213
Accession # Q9Y6Q6

Specificity

Detects human RANK in direct ELISAs and Western blots. In these formats, approximately 30% cross‑reactivity with recombinant mouse RANK is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human RANK/TNFRSF11A Antibody

Human RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation antibody in Mouse Splenocytes.

Human RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation in Mouse Splenocytes.

Human RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody induces osteoclast differentiation in the presence of 20 ng/mL recombinant mouse M-CSF (Catalog # 416-ML), in a dose-dependent manner, as measured by the tartrate-resistant acid phosphatase (TRAP) solution assay. The ED50 for this effect is typically 4-8 µg/mL.

Applications for Human RANK/TNFRSF11A Antibody

Application
Recommended Usage

Agonist Activity

4-8 µg/mL
Sample: Mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human RANK/TNFRSF11A Fc Chimera (Catalog # 683-RK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 5 publications using AF683 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References

  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.

Long Name

Receptor Activator of NF-kappa-B

Alternate Names

CD265, ODFR, TNFRSF11A, TRANCE R

Entrez Gene IDs

8792 (Human); 21934 (Mouse)

Gene Symbol

TNFRSF11A

UniProt

Additional RANK/TNFRSF11A Products

Product Documents for Human RANK/TNFRSF11A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RANK/TNFRSF11A Antibody

For research use only

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