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Human RANK/TNFRSF11A Antibody

Catalog # MAB683 | R&D Systems, Inc. a Bio-Techne Brand
Clone 80704 was used by HLDA to establish CD designation
Discontinued Product
MAB683 has been discontinued. View all RANK/TNFRSF11A products.

Key Product Details

Species Reactivity



CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Western Blot



Antibody Source

Monoclonal Mouse IgG1 Clone # 80704

Product Summary for Human RANK/TNFRSF11A Antibody


Mouse myeloma cell line NS0-derived recombinant human RANK/TNFRSF11A
extracellular domain
Accession # Q9Y6Q6


Detects human RANK/TNFRSF11A in ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymphotoxin beta R, rhNGF R, rhOPG, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse (rm) OX40, and rmRANK is observed.







Scientific Data Images for Human RANK/TNFRSF11A Antibody

Detection of RANK/TNFRSF11A antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of RANK/TNFRSF11A in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB683, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human RANK/TNFRSF11A Antibody

Recommended Usage


Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human RANK/TNFRSF11A Fc Chimera (Catalog # 683-RK)

Human RANK/TNFRSF11A Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human RANK/TNFRSF11A Biotinylated Antibody (Catalog # BAF683)
  • Standard: Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF (Catalog # 683-RK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage


Protein A or G purified from hybridoma culture supernatant


Reconstitute at 0.5 mg/mL in sterile PBS.


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.


  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.

Long Name

Receptor Activator of NF-kappa-B

Alternate Names


Entrez Gene IDs

8792 (Human); 21934 (Mouse)

Gene Symbol



Product Documents for Human RANK/TNFRSF11A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RANK/TNFRSF11A Antibody

For research use only

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