Human RANK/TNFRSF11A Antibody

Name: Human RANK/TNFRSF11A Antibody
Brand: R&D Systems
SKU: MAB683
Price: 159 EUR

Catalog # MAB683 | R&D Systems, Inc. a Bio-Techne Brand

Citations (9)

Product Datasheet / COA / SDS

Clone 80704 was used by HLDA to establish CD designation

Discontinued Product

MAB683 has been discontinued. View all RANK/TNFRSF11A products.

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 80704

Product Summary for Human RANK/TNFRSF11A Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human RANK/TNFRSF11A
extracellular domain
Accession # Q9Y6Q6

Specificity

Detects human RANK/TNFRSF11A in ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymphotoxin beta R, rhNGF R, rhOPG, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse (rm) OX40, and rmRANK is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human RANK/TNFRSF11A Antibody

Detection of RANK/TNFRSF11A in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB683, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human RANK/TNFRSF11A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: A431 human epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human RANK/TNFRSF11A Fc Chimera (Catalog # 683-RK)

Human RANK/TNFRSF11A Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human RANK/TNFRSF11A Biotinylated Antibody (Catalog # BAF683)
Standard: Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF (Catalog # 683-RK)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References

Anderson, D.M. et al. (1997) Nature 390:175.
Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.

Long Name

Receptor Activator of NF-kappa-B

Alternate Names

CD265, ODFR, TNFRSF11A, TRANCE R

Entrez Gene IDs

8792 (Human); 21934 (Mouse)

Gene Symbol

TNFRSF11A

UniProt

Q9Y6Q6

Product Documents for Human RANK/TNFRSF11A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human RANK/TNFRSF11A Antibody

For research use only

Citations for Human RANK/TNFRSF11A Antibody (9)

Showing 1 - 9 of 9 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human RANK/TNFRSF11A Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

KL Clar et al. (2021-12-04), Receptor Activator of NF-kappaB (RANK) Confers Resistance to Chemotherapy in AML and Associates with Dismal Disease Course Cancers, 2021-113(23).
PMID: 34885231
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Y Wang et al. (2018-07-05), Caveolin?1 enhances RANKL?induced gastric cancer cell migration Oncol. Rep., 2018-00(0).
PMID: 30015970
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Chypre M et al. (2016-01-07), Characterization and application of two RANK-specific antibodies with different biological activities. Immunol Lett, 2016-0171(0):5-14.
PMID: 26773232
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Schoppl A et al. (2015-02-24), Langerhans cell precursors acquire RANK/CD265 in prenatal human skin. Acta Histochem, 2015-0117(4):425-30.
PMID: 25722033
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Schmiedel B et al. (2012-12-14), Receptor activator for NF-kappaB ligand in acute myeloid leukemia: expression, function, and modulation of NK cell immunosurveillance. J Immunol, 2012-1190(2):821-31.
PMID: 23241893
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Schmiedel B et al. (2012-11-08), RANKL expression, function, and therapeutic targeting in multiple myeloma and chronic lymphocytic leukemia. Cancer Res, 2012-173(2):683-94.
PMID: 23139212
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Zhao E et al. (2012-03-01), Regulatory T cells in the bone marrow microenvironment in patients with prostate cancer. Oncoimmunology, 2012-01(2):152-161.
PMID: 22720236
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC

Barbaroux JB et al. (2008-07-15), Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation. J. Immunol., 2008-0181(2):1103-8.
PMID: 18606662
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Vernal R et al. (2006-10-01), High expression levels of receptor activator of nuclear factor-kappa B ligand associated with human chronic periodontitis are mainly secreted by CD4+ T lymphocytes. J. Periodontol., 2006-177(10):1772-80.
PMID: 17032122

There are no citations that match your criteria.