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Mouse IgG1 Isotype Control Best Seller

Catalog # MAB002 | R&D Systems, Inc. a Bio-Techne Brand
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MAB002

Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 11711

Product Summary for Mouse IgG1 Isotype Control

Immunogen

KLH

Specificity

Selected for use as mouse IgG1 isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT-II, rhBLC/BCA-1, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta8-1, recombinant viral (rv) CMV UL146, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin-2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhI-309, rhI-Linck, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin-1, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4,rmMCP-5, rvMCV type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rhMIP-1 delta, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha,rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhPARC, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTARC, rmTARC, rmTCA-3, rhTeck, or rmTeck.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Mouse IgG1 Isotype Control

Detection of Mouse IgG1Isotype Isotype Control by Flow Cytometry

Detection of Mouse IgG1Isotype Control by Flow Cytometry

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human LILRA6/CD85b Monoclonal Antibody (Catalog # MAB8656, filled histogram) or Mouse IgG1Isotype Control Antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, IGHG1, Igh-4, RGD1359539, VH7183

Product Documents for Mouse IgG1 Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IgG1 Isotype Control

For research use only

Citations for Mouse IgG1 Isotype Control (252)

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Customer Reviews for Mouse IgG1 Isotype Control (22)

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  • Mouse IgG1 Isotype Control
    Name: Lu Bai
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 10/11/2022
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/18/2021
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 05/05/2021
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 01/24/2021
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: K562 human chronic myelogenous leukemia cell line
    Species: Human
    Verified Customer | Posted 10/06/2020
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/08/2020
    used as a negative control
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Abby Sukarto
    Application: Flow Cytometry
    Sample Tested: Bone Extracts
    Species: Human
    Verified Customer | Posted 02/13/2020
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Mouse Breast Cancer Cells
    Species: Mouse
    Verified Customer | Posted 01/15/2020
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: SVEC4-10 mouse vascular endothelial cell line
    Species: Mouse
    Verified Customer | Posted 01/06/2020
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 12/08/2019
  • Mouse IgG1 Isotype Control
    Name: Abby Sukarto
    Application: Flow Cytometry
    Sample Tested: Bone Extracts
    Species: Human and Mouse
    Verified Customer | Posted 09/10/2019
    Need optimization of your specific cell types and use the same amount as the primary antibody.
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Affinity Purification
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 08/09/2019
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: SK-BR-3 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 10/23/2018
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 05/20/2018
    We used this as an Isotype Control for our Endothelial Chemotaxis Assay experiments. Conditioned Medium from Breast cancer cells mixed with IgG1 Isotype Control was used to compare Conditioned Medium containing cytokines (IL6, IL8, IL1B) neutralization antibody cocktail. Cancer Res; 78(5) March 1, 2018 (Fig 6F)
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 03/23/2018
  • Mouse IgG1 Isotype Control
    Name: Tao Huang
    Application: Block/Neutralize
    Sample Tested: IgG1
    Species: Mouse
    Verified Customer | Posted 10/20/2017
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Blood-derived monocytes
    Species: Human
    Verified Customer | Posted 08/01/2017
  • Mouse IgG1 Isotype Control
    Name: A G
    Verified Customer | Posted 05/26/2017
    Used in ICC with dog blood cell on a slide.
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Qing Zhang
    Application: Immunoprecipitation
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 05/16/2017
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Beta TC-6 mouse beta cell insulinoma cell line
    Species: Mouse and Human
    Verified Customer | Posted 08/17/2016
    PFA 4% fixation. Used at 94ug/mL. No signal until 120-250msec of acquisition time.
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: uman primary monocytes
    Species: Human
    Verified Customer | Posted 04/21/2016
  • Mouse IgG1 Isotype Control
    Name: SOjan Abraham
    Application: Flow Cytometry
    Sample Tested: Peripheral blood lymphocytes (PBL)
    Species: Human
    Verified Customer | Posted 01/21/2016

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