Your Complete Solution for Targeted Protein Degradation
Bio-Techne offers a full range of products and services for Targeted Protein Degradation research and development, including: small molecule Protein Degraders (e.g. PROTAC® degraders); aTAG and dTAG Degraders plus custom TAG knock-in cell lines; Degrader Building Blocks, Ubiquitin-Proteasome System Proteins and Assays; Assays for Protein Degradation and Custom Degrader Services.
Products and Services for Targeted Protein Degradation
Target Validation and Exploration
Target validation, the process of establishing that a potential molecular target is involved in a physiological function or disease process, is a key step in the development of new Degraders, as well as for drug discovery and biological research. Our TAG Degradation platform offers a differentiated method for target validation, using targeted protein degradation to reversibly knockdown a potential protein target and enable exploration of the downstream phenotype.
Degrader Design and Synthesis
Targeted protein degradation can be achieved using small molecule Protein Degraders, such as PROTAC® molecules. Bio-Techne provides a toolbox of products and services to support your Degrader Design and Synthesis program and save you time and money, including our PROTAC® Panel Builder online tool; a large range of off-the-shelf Degrader Building Blocks; and tools for new E3 ligase ligand discovery.
Assays for Targeted Protein Degradation
Successful development of small molecule Protein Degraders for targeted protein degradation requires an array of assays to address a specific and differentiated set of questions aimed at characterizing aspects of Degrader activity. The assay workflow should explore: target engagement and ternary complex formation induced by the Degrader; ubiquitination of the target protein; as well as target degradation and its downstream consequences. Multiple orthogonal assays will maximize the chance of successful Degrader development.
E3 Ligase and Ubiquitin Proteasome System Biology
Bio-Techne through the R&D SystemsTM brand provides a wide range of products to help you explore and characterize ubiquitin biology. The rapidly expanding portfolio includes highly active E3 Ubiquitin Ligases, including SKP2, VHL, Cereblon (CRBN) and DCAF proteins, as well as assay kits and components, antibodies and Tandem Ubiquitin Binding Entities (TUBEs). We also provide Cullin-Rbx complexes (including neddylated cullins) that can be coupled to your purified substrate binding complexes to generate active ligases.
Custom Services for Targeted Protein Degradation
Bio-Techne offers a unique portfolio of custom services to support your PROTAC® discovery and development and target validation programs. Harness our expertise in custom chemistry to develop active degraders to knock down your target of choice or custom building block panels. We also offer custom E3 ligase development services enabling you to build your own assays to investigate ubiquitination, and custom knock-in cell lines expressing your target protein of interest as a fusion with a "TAG" protein for use within our TAG degradation platform.
Bio-Techne 提供活性降解剂分子，包括 PROTAC 降解剂和 TAG 降解剂 (dTAG/aTAG)。PROTAC 分子包含一种与靶标蛋白（已通过化学链接与 E3 连接酶配体相连）结合的配体。降解剂同时结合靶标蛋白和 E3 连接酶，形成一个三元复合物，使靶标蛋白被泛素化，从而被蛋白酶体降解。TAG 降解剂的工作原理类似，但 E3 连接酶配体是连接在某一化合物上，该化合物可结合 FKBP12F36V（dTAG 系统）或 MTH1（aTAG 系统）突变体与靶标蛋白形成的融合蛋白。这些降解剂能够敲减没有已知配体的蛋白质。
Bio-Techne 提供一系列支持靶向蛋白降解研究的其他产品和服务，包括帮助您创建降解剂的降解剂构建单元，以及泛素-蛋白酶体系统 (UPS) 蛋白和分析、蛋白降解分析和定制降解剂服务。
PROTAC® 是 Arvinas Operations, Inc.的注册商标，经许可使用。