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Simple Western 仪器

Jess Simple Western instrument by ProteinSimple, a Bio-Techne brand

Jess

分析类型:对最多 25 个样本进行大小测定

检测模式:化学发光和荧光

ProteinSimple instrument Abby for chemiluminescence assays.

Abby

自动蛋白质印迹分析 分析类型:对最多 25 个样本进行大小测定 包括 RePlex™ 检测模式:化学发光  

Simple Western consumables by ProteinSimple a Bio-Techne brand

耗材

Simple Western 耗材:试剂、试剂盒和模块。

与 Jess™、Wes™、Abby™、Peggy Sue™、Sally Sue™ 和 NanoPro™ 1000 兼容

Peggy Sue Simple Western instrument ProteinSimple, a Bio-Techne brand

Peggy Sue

分析类型:对最多 96 个样本进行大小和电荷分析

检测模式:化学发光

Sally Sue Simple Western instrument ProteinSimple, a Bio-Techne brand

Sally Sue

分析类型:对最多 96 个样本进行大小分析

检测模式:化学发光

Nanopro 1000 Simple Western instrument ProteinSimple, a Bio-Techne brand

NanoPro 1000

分析类型:对最多 96 个样本进行电荷分析

检测模式:化学发光

Wes™ has been discontinued as of July 30th, 2021. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. Upgrade to Jess™ or Abby™ to have all the functionality of Wes with additional features like RePlex™ and Stellar™ High Sensitivity NIR/IR Detection (Jess only).

What is Simple Western?

Simple Western Brings the Western Blot Technique into the 21st Century

Simple Western™ from ProteinSimple, a Bio-Techne brand, is a next-generation biomolecular analytical tool that seamlessly combines capillary electrophoresis and immunodetection with conventional Western blot antibodies. Simple Western is the only fully automated Western blot system on the market, and it is completely gel-free, blot-free, and hands-free. With Simple Western, traditional Western blot steps like separation, washing, and detection are automatically performed inside a benchtop capillary electrophoresis machine. Just load as little as 3 µL of sample and come back to highly quantitative and reproducible results in just 3 hours.

More Than Just a Western Blot Alternative

With Simple Western, you can multiplex to get the most data per sample with industry-leading detection sensitivity in both NIR/IR fluorescence and chemiluminescence channels and normalize your protein expression data with built-in total protein normalization. Featured in hundreds of scientific publications and counting, Simple Western is a proven capillary Western blot technology that not only streamlines workflows, but also generates quantitative, reproducible, and publication-ready results that raise the bar on scientific rigor in protein analysis.

Simple Western Separates Proteins by Size or Charge

  1. Size-based capillary Western blot assays use capillary electrophoresis sodium dodecyl sulfate (CE-SDS) to separate proteins between 2 kDa and 440 kDa to analyze small polypeptides and large proteins like dystrophin to study Duchenne muscular dystrophy
  2. Charge-based capillary Western blot assays use capillary isoelectric focusing (cIEF) to separate proteins by their isoelectric points which provides a high-resolution characterization of protein isoforms and post-translational modifications like phosphorylation.
    Simple Western Size and Charge Separation Protein Assays Figure
    Simple Western automated capillary Western blot analysis

    How Can Simple Western Help You?

    Low Sample Volume, High Detection Sensitivity. Simple Western systems use as little as 3 µL of starting material and give down to picogram-level sensitivity. Up to 8 data points can be generated out of just 5 µL, allowing you to get the most out of your precious sample. Get even more data per sample with multiplex analysis in multicolor detection channels. 

    Fast Time to Results. Process 25 samples in just 3 hours, or 96 samples overnight, all in a fully-automated, walk-away fashion. 

    Gel-Free, Blot-Free, Hands-Free Automation. Simple Western assays are fully automated with no gels, no transfer devices, no blots, no film, and no manual analysis. Just load your samples and press start—it's a complete, walk-away capillary Western blot machine for protein separation and detection. Reclaim your time!

    Reproducible and Quantitative Data. By eliminating the variability inherent in manual traditional western blotting workflows, Simple Western assays are highly reproducible, while adding quantitation, and improving on time-to-result and overall data reliability. Abandon unreliable housekeeping controls and use Simple Western’s built-in total protein detection assays instead, so you can normalize your protein expression data with confidence and meet journal standards with publication-ready data.

    simple western advantage

    Industry-Leading Fluorescence Sensitivity for Western Blot Analysis with Stellar

    Now NIR/IR fluorescence detection on Simple Western is even better with Stellar™ NIR/IR Detection Modules for Jess™, which provides industry-leading fluorescence sensitivity at detection levels below 1 pg, along with excellent reproducibility and a 4-log dynamic range. With this leap in sensitivity, Stellar fluorescence detection rivals the widely recognized sensitivity of chemiluminescence detection and demolishes the fluorescence detection of the closest competing traditional Western blot imaging technology, which requires at least 50 pg.

    Industry-leading NIR/IR fluorescence detection sensitivity with Stellar

    Rethink, Reuse, RePlex

    Perform sequential immunoassays in the same capillary using RePlex™ with Jess™ and Abby™ and eliminate the strip-and-reprobe of traditional Western blots. RePlex removes the antibodies from the first round of probing to perform either a second round of probing with new antibodies or total protein detection. Importantly, RePlex efficiently removes antibodies between probing without compromising the integrity of the immobilized protein or its epitopes, allowing for excellent reproducibility across cycles. With Jess’s chemiluminescence and fluorescence channels, you can detect multiple targets per cycle. The second cycle can also be dedicated to total protein detection so that you can normalize your protein expression data with confidence. 

    Jess, Abby and Replex GIF

    哪个系统适合您?

    所有系统的操作条件均为:相对湿度 20-60%、非冷凝;温度范围 18-24℃;电压范围 100-230 V
    Simple Western 系统

    Jess

    Wes

    Sally Sue

    Peggy Sue

    NanoPro 1000

    Simple Western 大小分析

    Simple Western 电荷分析

    化学发光

    荧光

    每次运行的最大样本数

    25

    25

    96

    96

    96

    最大样本数时的运行时间(大小)

    <3 小时

    <3 小时

    14-19 小时

    14-19 小时

    不适用

    最大样本数时的运行时间(电荷)

    不适用

    不适用

    不适用

    11 小时

    11 小时

    样本冷却(大小)

    不适用

    不适用

    10ºC

    10ºC

    不适用

    样品冷却(电荷)

    不适用

    不适用

    不适用

    3ºC

    3ºC

    尺寸(高 x 宽 x 深)

    33 x 33 x 52 cm

    33 x 33 x 52 cm

    94 x 94 x 61 cm

    94 x 94 x 61 cm

    94 x 94 x 61 cm

    部件号

    004-650

    004-600

    004-700

    004-900

    004-109

    *批间 CV 具有系统控制,**峰面积百分比,***Jess/Wes 具有 HDR 检测模式。
     

    免疫分析技术规格(大小)

    免疫分析技术规格(大小)

    免疫分析技术规格(电荷)

    说明

    化学发光

    荧光

    化学发光

    所需样本

    0.3-1.2 µg 裂解物

    0.6-1.2 µg

    0.6-1.2 µg

    大小或 pI 范围

    2-40、12-230 和 66-440 kDa

    2-40、12-230 和 66-440 kDa

    最宽梯度范围为 pI 3 至 pI 10

    大小分析 CV

    <10%

    <10%

    <10%

    批内 CV

    <15%

    <15%

    <20%

    批间 CV

    <20%*

    <20%*

    <20%**

    分辨率(±分子量百分比差异)

    分子量 <20 kDa 时 ±15-20%

    分子量 >20 kDa 时 ±10%

    分子量 <20 kDa 时 ±15-20%

    分子量 >20 kDa 时 ±10%

    ±0.1 个 pI 单位

    定量 CV

    <20%

    <20%

    <20%

    动态范围

    2-3 个 log

    最多 4 个 log***

    3 个 log

    灵敏度

    ng

    低 pg

    低 pg

    毛细管

    5 cm,100 µm,400 nL

    5 cm,100 µm,400 nL

    5 cm,100 µm,400 nL

    Simple Western 应用

    Simple Western applications in cancer research

    癌症和免疫肿瘤学

    利用新一代蛋白质印迹工具,从样本中获取更丰富的信息,为您的癌症和免疫肿瘤学研究带来重大转变。

    sars cov-2 virus

    SARS-CoV-2

    使用自动化 Simple Western 系统,缩短您的实验室时间,加速您的 COVID-19 研究。您只需要设置好仪器,就可离开,然后 3 小时内即可远程查看分析数据!

    DNA editing gene therapy

    细胞和基因疗法

    新一代分析仪器,有助于改善和加速您的细胞和基因疗法工作流程。

    Targeted Degradation

    靶向降解

    使用无需手动操作的高通量 Simple Western 系统进行定量分析,促进您的筛选研究。

    SARS-CoV-2 Serology

    SARS-CoV-2 血清学

    只需一次运行,3 个小时,就能定量测定血清或血浆中对 5 种关键病毒抗原具有反应性的人 IgG 抗体!

    Exosome

    外泌体

    经过验证的细胞外囊泡样本蛋白质分析工作流程,由外泌体分离和高灵敏度蛋白质印迹解决方案领域的领先品牌提供。

    Vaccine

    Vaccine Development

    Improve reproducibility, quantitation, and time to market for your vaccine candidates.

    Neuroscience

    Neuroscience

    Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.

    Solutions for Cell Signaling and Signal Pathways Analysis

    Cell Signaling

    Analyze entire signaling pathways with high sensitivity multiplexing and total protein normalization.