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Simple Western Antibody Database

Find Antibodies Validated for Simple Western

Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.

How do antibodies become validated for Simple Western?

  • R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
  • Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
  • Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.

You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.

*Antibodies validated and backed by Cell Signaling Technology (CST)

Simple Western Validated Antibodies


Filter Antibodies: (5062)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
α 1-Acid Glycoprotein Primary R&D Systems
AF3694
Datasheet Goat Human serum Size 5 µg/mL 79
α 1-Microglobulin Primary R&D Systems
MAB7720
Datasheet Mouse Rat plasma Size 20 µg/mL 37
α 1B-Glycoprotein Primary R&D Systems
AF7757
Datasheet Sheep Human liver tissue Size 10 µg/mL 94
α 1B-Glycoprotein Primary R&D Systems
AF8537
Datasheet Rabbit Mouse plasma and rat plasma Size 1:100 113
α 2-Macroglobulin Primary R&D Systems
AF1938
Datasheet Goat Human serum Size 1 µg/mL 178
α 2-Macroglobulin Primary R&D Systems
MAB1938
Datasheet Mouse Human serum Size 10 µg/mL 178
α fodrin Primary Enzo Life Sciences
BML-FG6090
α-Fodrin Mouse Cell lysate, BAL fluid, plasma, serum Size 1:10, 1:50 150, 250
α Synuclein Primary Novus Biologicals
NBP1-05194
Datasheet Mouse Human Brain lysate 0.05 mg/ml Size 1:2000 20
α Synuclein Primary BD Biosciences
610786
Mouse Mouse striatum, mouse adrenal, rat adrenal, rat striatum Size 1:25 21
α Synuclein Primary BD Biosciences
610787
Mouse Size 1:10 12,20
α tubulin Primary Novus Biologicals
NB100-690
Citations (4)
Datasheet
Mouse HeLa lysate 1.0 mg/ml, Titrated to saturation using various models Size 1:50, 6 µg/mL 55
α Tubulin Primary Abcam
ab4074
Citations (1)
Datasheet
Rabbit NULL Size 1:1000 53
α Tubulin 4a Primary Novus Biologicals
NB120-11304
α Tubulin 4a (B-5-1-2) Datasheet Mouse Caco2 cell lysates Size 1:50
α-actinin Primary Santa Cruz Biotechnology
sc-17829
Unmodified Citations (1)
Datasheet
Mouse NCI-H1975 Size
α-Cardiac Actin Primary R&D Systems
MAB9308
Datasheet Mouse Human, mouse, and rat heart tissue Size 2 µg/mL 50
α-Cardiac Actin Primary R&D Systems
MAB93081
Datasheet Mouse Human Heart Size 1:20 49
α-Fetoprotein/AFP Primary R&D Systems
AF1369
Datasheet Chicken HepG2 human hepatocellular carcinoma cell line Size 2 µg/mL 70
α-Fetoprotein/AFP Primary R&D Systems
MAB1368
α-Fetoprotein Datasheet Mouse HepG2 human hepatocellular carcinoma cell line Size 10 µg/mL 70
α-Fetoprotein/AFP Primary R&D Systems
MAB13692
α-Fetoprotein/AFP (189508) Mouse HepG2 and HepB3 lysate 0.2 mg/ml Size 5 µg/mL 67-68
α-Fetoprotein/AFP Primary Novus Biologicals
NB110-7961
Datasheet Mouse Size
α-Galactosidase A/GLA Primary R&D Systems
AF6146
α-Galactosidase A/GLA Datasheet Sheep MCF-7 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line 0.2 mg/mL Size 50 µg/mL 55
α-Internexin Primary Novus Biologicals
NB300-140
Datasheet Mouse SH-SY5Y lysate Size 1:200 140
α-Nucleocapsid Primary Novus Biologicals
NB100-56683
SARS Nucleocapsid Protein Datasheet Rabbit Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2) Size 25 mg/mL 60
α-Smooth Muscle Actin Primary R&D Systems
MAB1420
Datasheet Mouse iBJ6 human induced pluripotent stem cell line differentiated to cardiomyocytes Size 10 µg/mL 49
α-Smooth Muscle Actin (D4K9N)* Primary Cell Signaling Technology
19245
Human Specific Citations (2)
Datasheet
Rabbit Human Colon Size 1:10–1:50 48, 49
α-Synuclein Primary R&D Systems
AF1338
Datasheet Goat Sk-Mel-28 Size 1:10 21
α-Synuclein Primary Cell Signaling Technology
2642
Rabbit Size 1:25 21
α-synuclein Primary Proteintech
10842-1-AP
Unmodified Citations (1)
Datasheet
Rabbit Heart Size 1:100
α-Synuclein (E4U2F)* Primary Cell Signaling Technology
51510
Datasheet Rabbit COS-7 + HA-Stat3 Size 1:10–1:50 20
α-Synuclein (Syn204) Primary Cell Signaling Technology
2647
α-Synuclein (Syn204) Citations (1)
Datasheet
Mouse Spinal cord 3 ug/ul Size 1:10 22
α-Synuclein, Phospho Primary Cell Signaling Technology
23706
Phospho α-Synuclein (Ser129) (D1R1R) Rabbit Spinal cord 3 ug/ul Size 1:250 23
α-Synuclein, Phospho Primary Cell Signaling Technology
93706
Phospho α-Synuclein (Ser129) (D1R1R) Rabbit Spinal cord 3 ug/uL Size 1:250 23
α-Tubulin (11H10)* Primary Cell Signaling Technology
2125
Citations (4)
Datasheet
Rabbit COS-7 Size 1:10–1:50 55
α-Tubulin (DM1A)* Primary Cell Signaling Technology
3873
Citations (2)
Datasheet
Mouse SupM2 Size 1:10–1:50 55
αA Crystallin/CRYAA Primary R&D Systems
AF4848
Datasheet Goat Mouse eye lens tissue Size 1 µg/mL 28
αB Crystallin/CRYAB Primary R&D Systems
AF4849
Datasheet Goat Human heart tissue and mouse heart tissue Size 2 µg/mL 29
αB Crystallin/CRYAB Primary R&D Systems
MAB4849
Datasheet Mouse Human heart tissue Size 1 µg/mL 28
β 2 Microglobulin Primary Abcam
ab75853
Unmodified Citations (2)
Datasheet
Rabbit Many Charge 1:50
β actin Primary Novus Biologicals
NB100-56874
Datasheet Rabbit HeLa lysate 1.0 mg/ml Size 1:12.5 49
β actin Primary Novus Biologicals
NB600-503
Citations (1)
Datasheet
Rabbit HeLa lysate 0.1 mg/ml Size 1:12.5 50
β actin Primary Novus Biologicals
NBP1-47423
Citations (1)
Datasheet
Mouse HeLa lysate 1.0 mg/ml Size 12.5 49
β Actin Primary Abcam
ab6276
β actin Citations (7)
Datasheet
Mouse Blood Size 1:25–1:100
β Actin Primary Abcam
ab8224
β actin Citations (3)
Datasheet
Mouse Blood Size 1:100 49
β Actin Primary Abcam
ab8226
Cytoskeleton Citations (1)
Datasheet
Mouse HeLa, Jurkat, HEK 293 Size 1:50 48
β actin Primary Proteintech
HRP-60008
Mouse HeLa cell lysates Size 1:50 47
β Adducin Primary Novus Biologicals
NBP2-33974
β Adducin Datasheet Rabbit Size
β amyloid Primary Cell Signaling Technology
2454
Rabbit HeLa lysate spiked with b-amyloid Size 1:100 3
β amyloid Primary BioLegend
803015
β amyloid amino acid position 1-16 Mouse Cerebro Spibal Fluid (CSF) peptides Charge 1:500
β arrestin Primary Novus Biologicals
NBP2-24569
Datasheet Rabbit Jurkat lysate 0.5 mg/ml Size 1:100 54
β catenin Primary Novus Biologicals
NBP1-54467
Citations (1)
Datasheet
Mouse HepG2 lysate 0.5 mg/ml Size 1:100 90

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