Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human 15-PGDH/HPGD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for 15-PGDH/HPGD at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human 15‑PGDH/HPGD by Simple Western^TM.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for 15-PGDH/HPGD at approximately 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Human 15-PGDH/HPGD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5660) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human 15-PGDH/HPGD Antibody

Application

Recommended Usage

Simple Western

10 µg/mL
Sample: Human liver tissue

Western Blot

1 µg/mL
Sample: Human liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 15-PGDH/HPGD

HPGD, or 15-hydroxyprostaglandin dehydrogenase, is a NAD⁺-linked dehydrogenase that oxidizes the hydroxyl group at position 15 of prostaglandins to a ketone, resulting in a loss of biological activity (1). HPGD is a major enzyme for the catabolism of prostaglandins. The enzyme is a member of the short-chain alcohol dehydrogenase family of enzymes (2). HPGD is a cytosolic enzyme expressed in most tissues, with highest expression levels in placenta, lung, and kidney (3). It is inhibited by aspirin and nonsteroidal anti-inflammatory drugs (4). Defects in HPGD are a cause of hypertrophic osteoarthropathy (5).

References

Anggard, E. and B. Samuelsson (1964) J. Biol. Chem. 239:4097.
Krook, M. et al. (1990) Biochemistry 29:738.
Tai, H.H. (1976) Biochemistry 15:4586.
Mak, O.T. et al. (1982) Biosci. Rep. 2:503.
Uppal, S. et al. (2008) Nat. Genet. 40:789.

Long Name

15-Hydroxyprostaglandin Dehydrogenase

Alternate Names

15PGDH, HPGD, PGDH1, SDR36C1

Entrez Gene IDs

3248 (Human); 15446 (Mouse); 79242 (Rat)

Gene Symbol

HPGD

UniProt

P15428 (Human)

Additional 15-PGDH/HPGD Products

Product Documents for Human 15-PGDH/HPGD Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human 15-PGDH/HPGD Antibody

For research use only

Citations
Reviews

Human 15-PGDH/HPGD Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5660

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all 15-PGDH/HPGD Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human 15-PGDH/HPGD Antibody

Detection of Human 15-PGDH/HPGD by Western Blot.

Detection of Human 15‑PGDH/HPGD by Simple WesternTM.