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Goat IgG Horseradish Peroxidase-conjugated Antibody
Catalog # HAF019 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Goat
Applications
Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Chicken IgY
Product Summary for Goat IgG Horseradish Peroxidase-conjugated Antibody
Immunogen
Goat IgG
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rabbit IgG and human IgG is observed.
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Scientific Data Images for Goat IgG Horseradish Peroxidase-conjugated Antibody
Detection of Goat IgG Primary Antibody by Western Blot.
Western blot shows detection of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Polyclonal Antibody (AF4920) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Vimentin in Human Kidney
Detection of Vimentin in human kidney using 5 ug/mL of Goat anti-human Vimentin primary antibody (AF2105) followed by 30 min incubation with Donkey Anti-Goat HRP conjugated secondary antibodies (Catalog# HAF019) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.Applications for Goat IgG Horseradish Peroxidase-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Use at a 1:20
dilution
Western Blot
Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using HAF019 in the following applications:
Published Applications
Read 21 publications using HAF019 in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified from egg yolks
Formulation
Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
IgG, IGHG1, Immunoglobulin G, ImmunoglobulinG
Product Documents for Goat IgG Horseradish Peroxidase-conjugated Antibody
Product Specific Notices for Goat IgG Horseradish Peroxidase-conjugated Antibody
For research use only
Citations for Goat IgG Horseradish Peroxidase-conjugated Antibody (21)
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Citations are publications that use Bio-Techne products. Selected citations for Goat IgG Horseradish Peroxidase-conjugated Antibody include:
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Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotZuo et al. (2023-06-15), Liver-specific in vivo base editing of Angptl3 via AAV delivery efficiently lowers blood lipid levels in mice Cell & bioscience
PMID: 37322547 -
Species: Mouse
Sample Types: Cell Lysates
Xu et al. (2023-06-12), Subfunctionalized expression drives evolutionary retention of ribosomal protein paralogs Rps27 and Rps27l in vertebrates eLife
PMID: 37306301 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotE Sucajtys-S et al. (2023-03-17), Hepatocyte Nuclear Factor-1alpha Increases Fibrinogen Gene Expression in Liver and Plasma Fibrinogen Concentration in Rats with Experimental Chronic Renal Failure International Journal of Molecular Sciences, 2023-024(6).
PMID: 36982805 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotD Langgartne et al. (2023-02-01), Mycobacterium vaccae protects against glucocorticoid resistance resulting from combined physical and psychosocial trauma in mice Brain, Behavior, and Immunity, 2023-0109(0):221-234.
PMID: 36736929 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotE Sucajtys-S et al. (2022-08-10), Hepatocyte Nuclear Factor 1alpha Proinflammatory Effect Linked to the Overexpression of Liver Nuclear Factor-kappaB in Experimental Model of Chronic Kidney Disease International Journal of Molecular Sciences, 2022-023(16).
PMID: 36012158 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotL Xiong et al. (2022-05-27), Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells Elife, 2022-011(0).
PMID: 35621159 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotYG Gete et al. (2021-06-04), Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS Aging Cell, 2021-00(0):e13388.
PMID: 34086398 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryKP Kim et al. (2021-04-12), Donor cell memory confers a metastable state of directly converted cells Cell Stem Cell, 2021-00(0).
PMID: 33848472 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCY Wang et al. (2020-11-10), Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats Drug Des Devel Ther, 2020-114(0):4825-4834.
PMID: 33204068 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotKP Kim et al. (2020-09-02), Reprogramming competence of OCT factors is determined by transactivation domains Science Advances, 2020-06(36).
PMID: 32917606 -
Species: Mouse
Sample Types: Plasma
Applications: ELISA DetectionS Zalba et al. (2020-06-26), Quantification of Pharmacokinetic Profiles of PD-1/PD-L1 Antibodies by Validated ELISAs Pharmaceutics, 2020-012(6).
PMID: 32604843 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotL Ou et al. (2020-01-20), Sialylation of FGFR1 by ST6Gal?I overexpression contributes to ovarian cancer cell migration and chemoresistance Mol Med Rep, 2020-021(3):1449-1460.
PMID: 32016470 -
Species: Rat
Sample Types: Whole Cells
Applications: Western BlotF Navid et al. (2018-03-25), The Role of Autophagy in the Degradation of Misfolded HLA-B27 Heavy Chains Arthritis & rheumatology (Hoboken, N.J.), 2018-00(0).
PMID: 29342507 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotX Miao et al. (2018-03-15), Thrombin-reduced miR-27b attenuates platelet angiogenic activities in vitro via enhancing platelet synthesis of anti-angiogenic thrombospondin-1 J. Thromb. Haemost., 2018-00(0).
PMID: 29442415 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PXP Zhang et al. (2017-10-23), Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma Oncol Lett, 2017-114(6):8183-8189.
PMID: 29250193 -
Species: Human
Sample Types: Plasma
Applications: Western BlotK Tang et al. (2017-03-23), Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity J Immunol Res, 2017-02017(0):6468097.
PMID: 28424792 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotE Casta¤o et al. (2017-01-19), Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels PLoS ONE, 2017-012(1):e0170389.
PMID: 28103309 -
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western BlotSester D et al. (2015-06-26), Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice. J Immunol, 2015-0195(3):1233-41.
PMID: 26116505 -
Species: Mouse
Sample Types: BALF
Applications: Western BlotLouten J et al. (2012-07-10), Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 2012-07(0):87-104.
PMID: 22837640 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotXavier S et al. (2010-09-24), BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. PLoS ONE, 2010-05(9):e12995.
PMID: 20886049 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotGreig JA et al. (2009-07-14), Influence of coagulation factor x on in vitro and in vivo gene delivery by adenovirus (Ad) 5, Ad35, and chimeric Ad5/Ad35 vectors. Mol. Ther., 2009-017(10):1683-91.
PMID: 19603000
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Customer Reviews for Goat IgG Horseradish Peroxidase-conjugated Antibody (1)
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