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干细胞分离、培养、分化和表征
Bio-Techne 为干细胞研究从分离和培养到确认、重新编程、分化以及表征的所有阶段提供支持。我们为细胞分离，针对细胞标记物表达、免疫分析、干细胞分化和单细胞分析的多色流式细胞术提供经优化和可重现的试剂盒。我们的优质试剂包括细胞因子和生长因子、细胞外基质蛋白、小分子、抗体、抗体组合和专用细胞培养基。
胚胎干细胞和诱导性多能干细胞
胚胎干细胞和诱导性多能干细胞
我们为您开展胚胎干细胞 (ES) 和诱导性多能干细胞 (iPSC) 研究提供众多工具，包括辐照小鼠胚胎成纤维细胞、小鼠胚胎成纤维细胞条件培养基、抗体标志物组合和培养基补充剂。
Serum-Free and Animal-Free Cell Culture
Increase the consistency of your cell cultures as you approach translational studies for regenerative medicine and cell therapy programs. Adopting these media will
- Reduce variability in media composition
- Simplify compliance with regulatory guidelines
- Simplify comparability testing for raw material changes
Why Use Bio-Techne Reagents for Culturing Stem Cells?
Our mission is to facilitate discovery and translation by ensuring high performance and lot-to-lot consistency of our products. Consistency is key to minimizing variability and standardizing protocols.
Don’t take it from us, listen as Stem Cell Core Facility Directors describe why they prefer Bio-Techne proteins and small molecules for their cultures!
Featured Products
Cultrex™ UltiMatrix RGF Basement Membrane Extract
Cultrex™ UltiMatrix RGF Basement Membrane Extract
- Designed for organoid and stem cell culture
- Protein composition and tensile strength optimized for 3D applications
- Qualified for dome formation
ExCellerate™ iPSC Expansion Medium
ExCellerate™ iPSC Expansion Medium
- Animal component-free
- Complete formulation – no growth factor supplementation required
- Stable over long term culture
Coming Soon
Animal-Free GMP BMP‑4
Animal-Free GMP BMP‑4
- Equivalent bioactivity to our other BMP-4 proteins
- Animal-free for clinical manufacturing processes
- Backed by our quality standards and facility certifications. See GMP Capabilities
Animal-Free Recombinant Proteins
Ensure a seamless transition from preclinical research into clinical manufacturing by starting your preclinical experiments with our Animal-Free RUO proteins. R&D SystemsTM Animal-Free RUO proteins are produced in dedicated animal-free laboratories and are manufactured using the same systems as our Animal-Free GMP-Grade proteins.
Custom Services for Stem Cell Research
The multitude of potential stem cell preparations and applications means that the exact product you need for your research may not be available. If you can’t find what you need, our expert scientists and custom services team are here to help. We will work with you to deliver reagents and assays to meet your requirements. Whether it’s as simple as a change in formulation or a completely new product, take advantage of our resources and expertise to overcome your challenge.
Streamlining Translation for Regenerative Medicine
When it’s time to advance your regenerative medicine product to clinical manufacturing, partner with us for reliability, quality, and custom services. We will work with you to provide reproducible production of reagents and assays at clinical scale, with complete documentation. We offer GMP reagents as well as 21 CFR Part 11-compliant analytical instruments for automation and high throughput. We’re committed to helping you streamline the manufacture of regenerative medicine products.
Website Resources
- Stem Cell Protocols at R&D Systems
- Protocols for Stem Cells at Tocris
- Stem Cells Markers Interactive Tool at R&D Systems
- Webinars
eBooks
- Technical Guide: Basement Membrane Basics at R&D Systems
- Cytokine and Protein User’s Guide Book at R&D Systems
- Evolution of Cell Culture Models eBook at R&D Systems
- Next-Generation Analytical Solutions For Cell & Gene Therapy eBook at ProteinSimple
- Stem Cell Analysis: Advances in Western Blotting Technologies eBook at ProteinSimple
Literature
- Embryonic and Induced Pluripotent Stem Cell Workflow Solutions Brochure at R&D Systems
- Stem Cell Workflow: Using Small Molecules Poster at Tocris
- Stem Cells and Differentiation Poster at Tocris
- Stem Cell Research Product Guide at Tocris
- Technical Notes: Assessing the Pluripotent Status of Stem Cells at R&D Systems
- Differentiation potential of iPSC at R&D Systems
- Mending the Myocardium: Tracking Cardiomyocyte Differentiation with Single-Cell Westerns at ProteinSimple
I have been using R&D / Bio-Techne perhaps for the last maybe 20 years, ever since I was a graduate student. My lab would exclusively use R&D products. I've found R&D / Bio-Techne to be extremely reliable.
The Bio-Techne products that we’re using, we love and what we're using are cytokines, growth factors and we have been using them since the beginning. I mean our core started in 2008 and we really have not changed.
So in our stem cell core facility, we do a lot of differentiation into different progenies. For all this differentiation we use a number of different cytokines, growth factors and we buy them all from R&D Systems or Tocris.
We had very good experience with R&D cytokines as well as antibodies. We didn't find any lot-to-lot variation with the cytokines and antibodies. So, in general, we're very pleased.
The product, the reproducibility has been consistent, and we've seen that everything works, everything is good, and now a lot of the products we buy and we don't lot test and we haven't had issues. Everything has been excellent and has worked beautifully.
About Stem Cells
The defining characteristics of stem cells, i.e. their ability to self-renew and differentiate, make them a valuable resource in medical research and therapy. Directed differentiation of stem cells can provide insights into embryonic development, generate organ-like structures (organoids), and help us understand complex disease processes.
Stem cells are unspecialized cells that are capable of self-renewal through mitotic cell division, even after long periods of inactivity. Stem cell differentiation gives rise to the endoderm, ectoderm, and mesoderm germ layers which develop into the specialized cells of a tissues and organs.
Embryonic stem cells (ESCs) are found in the inner cell mass of blastocysts, adult (or somatic) stem cells maintain the populations of specific cell types, induced pluripotent stem cells (iPSCs) are produced by the reprogramming of somatic cells, and cancer stem cells (CSCs) reside in tissues where they give rise to local tumors. Hematopoietic stem cells (HSC) differentiate into the myeloid and lymphoid immune cell lineages. Multipotent mesenchymal stem cells (MSC) give rise to adipocytes, chondrocytes, osteocytes, and cardiomyocytes. Neural stem cells (NSC) can differentiate into neurons, astrocytes, and oligodendrocytes.
Owing to their tissue regenerative potential, stem cells can be utilized to repair or replace old or damaged cells, and this property is valuable in regenerative medicine. Stem cells offer the potential for treating many diseases including cancer, neurodegeneration, musculoskeletal disorders, heart disease, and diabetes. 3-dimensional cultures and organoids based on differentiated stem cells reproduce some functions of native tissues and are valuable for toxicology studies and drug discovery.
Gene Engineering Services
Realize the potential of gene engineering by partnering with our experts. Engineer any cell type including T cells and iPSC with our non-viral TcBuster™ gene delivery system.
For cell and gene therapy manufacturing, we offer GMP CDMO services that include process development for cell engineering, quality control, custom cell vialing, and regulatory support.
