Tissue Clearing Pro-Organoid

Tissue Clearing Pro-Organoid is a rapid, easy-to-use, reversible and non-destructive kit, which allows effective tissue clearing and staining of organoids, 3D cell cultures and microtissues. Tissue Clearing Pro-Organoid is a solvent based tissue clearing technique, comparable to BABB and DISCO. Tissue Clearing Pro-Organoid does not cause significant shrinkage or tissue damage and does not use as harsh a solvent as other solvent based techniques.

This organoid tissue clearing kit allows 3D cell cultures up to 500 μm thick to be cleared in minutes, and is compatible with fluorescent protein, immunolabeling and small molecule staining techniques, as well as being suitable for use with all standard dyes and buffers. Following 3D imaging, Tissue Clearing Pro-Organoid can be reversed for follow-up 2D histology staining. The kit comes with a getting started guide and a more comprehensive tissue clearing guidebook, providing detailed protocols. Tissue Clearing Pro-Organoid kit contains 30 mL of Tissue Clearing Pro-Organoid clearing reagent plus the 4 buffer solutions required to process 3D cell cultures for immunolabeling and is enough to process three 96-well plates of material.

Please read the Tissue Clearing Pro-Organoid Guidebook for more information and detailed protocols for using this kit.