Organoid and 3-D Cell Culture Products

• Different formats of organoid-qualified Cultrex Basement Membrane Extracts are available to promote robust cell growth, viability, and culture consistency

• Our latest, most advanced Cultrex UltiMatrix RGF Basement Membrane Extract has been optimized to improve the performance and consistency of spheroid, organoid, and stem cell cultures

• Reduced growth factor formulations provide more defined culture systems

• Quality controlled for lot-to-lot performance consistency

• We offer a complete catalog of R&D Systems^TM proteins commonly used for organoid culture, including even the most difficult to express and purify proteins such as R-Spondin-1, Noggin, and Wnt-3a

• All of our recombinant proteins are rigorously tested for bioactivity and lot-to-lot consistency 

• Each new production lot of protein is tested to ensure that it meets our expectations for endotoxin specification, which is an industry-leading <0.1 EU/µg

• R&D Systems proteins are highly cited in organoid-related scientific publications and frequently display greater activity than leading competitors’ products

• Our recombinant proteins can be ordered in bulk at a discounted price and custom protein services are also available

• A large selection of Tocris^TM small molecules that are commonly used as 3-D growth matrix components and additives for long-term organoid growth are available

• Our catalog includes the ALK kinase inhibitor, A 83-01, the highly selective GSK-3 inhibitor CHIR 99021, the potent ROCK inhibitor, Y-27632 dihydrochloride, the p38 MAPK inhibitor, SB 202190, and many more

• We offer a variety of different formats of base media for organoid cultures, including media with or without glucose, L-glutamine, HEPES, NEAA, nucleosides, phenol red, or sodium pyruvate content

• We develop and manufacture serum-free media supplements to support organoid cultures

• Our catalog includes cryopreservation media, which is optimized to increase cell recovery and viability

• Our catalog includes over 780,000 R&D Systems™ and Novus Biologicals™ antibodies for more than 25 species

• We offer both unlabeled and labeled primary antibodies with 20 different potential fluorescent conjugates

• Our primary antibodies are validated for one or more of up to 15 applications

• We offer both conjugated and unconjugated secondary antibodies and a range of isotype control antibodies

• Our antibodies come in a variety of size options, including bulk and sample sizes to ensure that you can find what you need for your research

• Utilize this technology to spatially map and quantify specific target mRNAs in intact cells and tissues

• The assay can be combined with immunohistochemistry or immunofluorescence on the same slide to investigate mRNA and protein co-expression

• Up to 12 RNA targets can be detected simultaneously using the RNAscope HiPlex assay

• Catalog probes are available for over 23,000 targets in 140+ species and can also be made-to-order

• RNAscope ISH Assays are cited in over 2,600 publications across multiple different research areas

To assist researchers, R&D Systems cell culture scientists are working every day to optimize the culture conditions for growing different types of organoids. As we see success, the recipes and protocols are being published on the Organoid and 3-D Cell Culture Protocols page of our website.

Watch Bio-Techne’s Virtual Organoid Symposium from May 2020 to learn more about how leading scientists are utilizing organoids in their research. Presenters include Mr. Jens Puschof and Dr. Talya Dayton from Dr. Hans Clevers lab at the Hubrecht Institute in the Netherlands, and Dr. Robert Barrett from Cedars Sinai Medical Center in Los Angeles, CA.

Download this eBook to learn about the history, current state, and future use of cell-based 2-D and 3-D model systems for studying disease conditions and drug screening. Key topics addressed are the use of cell lines and primary cells for toxicology, pluripotent stem cells models, 2-D vs. 3-D cell culture, the benefits and challenges of organoid models, and toxicology assays.

Explore the data presented in this scientific poster to learn about the features of Cultrex UltiMatrix RGF Basement Membrane Extract that make it an ideal matrix for organoid cultures. This piece includes competitor comparison data showing that UltiMatrix Basement Membrane Extract has more consistent protein concentrations and gelling rates across lots than the leading commercial basement membrane extract.

Browse our collection of products for cell culture. We offer a comprehensive range of reagents to promote robust cell growth, including media and supplements, FBS, basement membrane extracts, recombinant proteins, and custom cell culture services.

Minimize variability in your stem cell experiments and ensure high performance by using our stem cell culture reagents. From isolation and culture to verification, reprogramming, differentiation, and characterization, we have products that you need for your stem cell work.