DAPI
Tocris Bioscience, part of Bio-Techne | Catalog # 5748
Blue-fluorescent DNA stain / dye. Used for: nuclear counterstain, fixed and live-cell staining, assessing apoptosis. Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), ISH
Key Product Details
Description
Blue-fluorescent DNA stain / dye. Used for: nuclear counterstain, fixed and live-cell staining, assessing apoptosis. Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), ISH
Alternative Names
DAPI Dye
Product Description
Key information: DAPI is a widely used blue-fluorescent DNA stain / dye. Membrane impermeant to live cells. For nuclear counterstain, fixed and live-cell staining (at high concentration).Used for: nuclear counterstain, fixed and live-cell staining (at high concentration), DNA visualization, chromosome staining, DNA staining in agarose gels, apoptosis analysis, mouse embryos or fetal organs visualization.
Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), and ISH.
Properties and Photophysical Data: DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 350 nm and 470 nm, respectively.
Scientific Data Images for DAPI
DAPI staining.
HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm.
DAPI counterstaining alongside MitoBrilliant™ Live 646.
HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm.
RNAScope™ Assay with DAPI Counterstain.
3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with ClariTSA™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).Optical Data
| Emission Color | Blue |
| λabs | 350 nm |
| λem | 470 nm |
| Closest Laser line | 405 nm |
| Application | Flow Cytometry, ISH, Confocal Microscopy, Immunofluorescence (IHC, ICC) |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Product Specifications
Molecular Weight
350.25
Formula
C16H15N5.2HCl
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
CAS Number
28718-90-3
PubChem ID
160166
InChI Key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
SMILES
N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.5 | 10 with gentle warming | |
| DMSO | 35.02 | 100 |
Preparing Stock Solutions
for DAPI
The following data is based on the product molecular weight 350.25
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.86 mL | 14.28 mL | 28.55 mL |
| 5 mM | 0.57 mL | 2.86 mL | 5.71 mL |
| 10 mM | 0.29 mL | 1.43 mL | 2.86 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.57 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Sandell DAPI staining of whole-mount mouse embryos or fetal organs. Cold Spring Harb.Protoc. 2018 PMID: 30275072
- Kapuscinski DAPI: a DNA-specific fluorescent probe. Biotech.Histochem. 1995 PMID: 858020
- Mildner Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 1978 PMID: 348301
- Chazotte Labeling nuclear DNA using DAPI. Cold Spring Harb.Protoc. 2011 PMID: 21205856
- Russell A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 1975 PMID: 46112
Product Specific Notices for DAPI
For research use only
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