Y-27632 dihydrochloride

Tocris Bioscience, part of Bio-Techne | Catalog # 1254

Selective ROCK inhibitor; inhibits ROCK1 (p160 ROCK) and ROCK2; stem cell freezing (cryopreservation) media component
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Key Product Details

Description

Selective ROCK inhibitor; inhibits ROCK1 (p160 ROCK) and ROCK2; stem cell freezing (cryopreservation) media component

Alternative Names

y27632,Y-27632 2HCl,ROCK inhibitor,ROCK1 inhibitor

View all Rho-kinase Inhibitors »

Product Description

Y-27632 dihydrochloride is a selective ROCK inhibitor (Ki values are 0.14-0.22, 0.3, 25, 26 and > 250 μM for ROCK1 (p160 ROCK), ROCK2, PKA, PKC and MLCK respectively). Also inhibits PRK2 (IC50 = 600 nM). Increases survival rate of human embryonic stem (hES) cells and iPSC undergoing cryopreservation. Used in combination with CHIR 99021, RepSox, Forskolin, SP 600125, Go 6983 and VPA to reprogram fibroblasts to mature neurons. Also optimizes naive human pluripotent stem cell growth and viability following naive cell derivation from primed ESCs and iPSCs using naive human stem cell medium (NHSM). Used as component of growth media for urothelial organoids. Also used in a protocol to generate brain organoids from human iPSCs. Cell-permeable, orally bioavailable and active in vivo.

Y-27632 in solution and Y-27632 synthesized to cGMP guidelines also available.

For more information about how Y-27632 dihydrochloride may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts, Generation and Propagation of EPS cells (LCDM Cocktail), Generating Vascular Organoids, Generating Midbrain Dopaminergic Neurons from hPSCs, Cultivating Cerebral Organoids, 3D Culture of Lung Alveolar Cells.

Scientific Data Images for Y-27632 dihydrochloride

ROCK inhibition by Y-27632 and immunostaining of skeletal myogenic differentiation of MESP1+ mesoderm,

Application of Y-27632 dihydrochloride in MESP1+ Mesoderm.

ROCK inhibition by Y-27632 (1 μM) promotes skeletal myogenic differentiation of MESP1+ mesoderm, as shown by immunostaining for Myosin Heavy Chain+ (Green) and Myogenin (Red), and counterstaining with DAPI (Catalog # 5748; Blue, Tocris). Data courtesy of Dr Sunny Chan and Dr Michael Kyba, University of Minnesota.
iPSC-derived cerebral organoids

Application of Y-27632 dihydrochloride in Culture and Characterization of iPSC-derived Cerebral Organoids.

iPSC-derived cerebral organoids (day 45) were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (Catalog # BME001-05) and brain organoid culture medium, which includes Y-27632 dihydrochloride, along with other reagents listed in the brain organoid culture recipe. Cerebral organoids were stained for Syto6 (blue), Pax6 (red), and Vimentin (green). (A) Image taken at 4x magnification. (B) An enlarged view of the area shown within the white box in part A of the figure. (C) Image taken at 15x magnification. Images courtesy of LifeCanvas Technologies.
iPSC-derived cerebral organoids

Application of Y-27632 dihydrochloride in Culture and Characterization of iPSC-derived Cerebral Organoids.

iPSC-derived cerebral organoids (day 45) were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (Catalog # BME001-05) and brain organoid culture medium, which includes Y-27632 dihydrochloride, along with other reagents listed in the brain organoid culture recipe. (A) Representative brightfield image of day 30 iPSC-derived cerebral organoids. (B) Cerebral organoids stained for beta III-tubulin (green) and Prox1 (red), and counterstained with DAPI (blue) (Catalog # 5748).
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Product Specifications

Molecular Weight

320.26

Formula

C14H21N3O.2HCl

Storage

Desiccate at RT

Purity

≥98% (HPLC)

Chemical Name

trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride

CAS Number

129830-38-2

PubChem ID

14801906

InChI Key

IDDDVXIUIXWAGJ-LJDSMOQUSA-N

SMILES

N[C@H](C)[C@@]1([H])CC[C@H](C(NC2=CC=NC=C2)=O)CC1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 32.03 100
DMSO 32.03 100

Preparing Stock Solutions for Y-27632 dihydrochloride

The following data is based on the product molecular weight 320.26

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.12 mL 15.61 mL 31.22 mL
5 mM 0.62 mL 3.12 mL 6.24 mL
10 mM 0.31 mL 1.56 mL 3.12 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Y-27632 dihydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Y-27632 dihydrochloride

For research use only

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