HEPES

Name: HEPES
Brand: Tocris Bioscience
SKU: 3173
Rating: 5 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 3173

(3)

Citations (543)

COA / SDS

Multipurpose HEPES buffer; used in cell culture

Key Product Details

Description:	Multipurpose HEPES buffer; used in cell culture
Chemical Name:	4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Purity:	≥99.5%
Molecular Weight:	238.3

View Full Technical Details for HEPES

Technical Data
Citations / Reviews (3)

Biological Activity for HEPES

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media. HEPES can be used in a small molecule cocktail to generate 3D Culture of Lung Alveolar Cells (see our protocol below).

For more information about how HEPES may be used, see our protocol: 3D Culture of Lung Alveolar Cells

Technical Data for HEPES

M. Wt	238.3
Formula	C₈H₁₈N₂O₄S
Storage	Store at RT
Purity	≥99.5%
CAS Number	7365-45-9
PubChem ID	23830
InChi Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Smiles	OCCN1CCN(CCS(=O)(O)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for HEPES

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	238.3	1000

Preparing Stock Solutions for HEPES

The following data is based on the product molecular weight 238.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
10 mM	0.42 mL	2.10 mL	4.20 mL
50 mM	0.08 mL	0.42 mL	0.84 mL
100 mM	0.04 mL	0.21 mL	0.42 mL
500 mM	0.01 mL	0.04 mL	0.08 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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References for HEPES

References are publications that support the biological activity of the product.

Medzon and Gedies Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol.
Good Hydrogen ion buffers for biological research. Biochemistry 1966 PMID: 5942950