HEPES

Tocris Bioscience, part of Bio-Techne | Catalog # 3173

Multipurpose HEPES buffer; used in cell culture
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Key Product Details

Description

Multipurpose HEPES buffer; used in cell culture

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Product Description

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media. HEPES can be used in a small molecule cocktail to generate 3D Culture of Lung Alveolar Cells (see our protocol below).

For more information about how HEPES may be used, see our protocol: 3D Culture of Lung Alveolar Cells

Product Specifications

Molecular Weight

238.30

Formula

C8H18N2O4S

Storage

Store at RT

Purity

≥99.5%

Chemical Name

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

CAS Number

7365-45-9

PubChem ID

23830

InChI Key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

SMILES

OCCN1CCN(CCS(=O)(O)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 238.3 1000

Preparing Stock Solutions for HEPES

The following data is based on the product molecular weight 238.30

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
10 mM 0.42 mL 2.10 mL 4.20 mL
50 mM 0.08 mL 0.42 mL 0.84 mL
100 mM 0.04 mL 0.21 mL 0.42 mL
500 mM 0.01 mL 0.04 mL 0.08 mL

Calculators

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background References

References are publications that support the biological activity of the product.

  • Medzon and Gedies Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol.
  • Good Hydrogen ion buffers for biological research. Biochemistry 1966 PMID: 5942950

Product Documents for HEPES

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HEPES

For research use only

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