Nicotinamide

Name: Nicotinamide
Brand: Tocris Bioscience
SKU: 4106
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4106

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Citations (47)

COA / SDS

PARP-1 inhibitor

Key Product Details

Description:	PARP-1 inhibitor
Chemical Name:	Pyridine-3-carboxamide
Purity:	≥99% (HPLC)
Molecular Weight:	122.12

View Full Technical Details for Nicotinamide

Technical Data
Citations / Reviews (1)

Biological Activity for Nicotinamide

Nicotinamide is an anti-inflammatory agent. Inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. NAD⁺ precursor. Promotes differentiation of mesenchymal stem cells to insulin producing cells when used in combination with growth factors and high glucose concentration. Nicotinamide also acts as a SIRT1 inhibitor and promotes the expansion of hematopoietic progenitor cells. Nicotinamide can be used in a small molecule cocktail to generate 3D culture of lung alveolar cells.

Nicotinamide synthesized to Ancillary Material Grade also available.

For more information about how Nicotinamide may be used, see our protocol: 3D Culture of Lung Alveolar Cells

Compound Libraries for Nicotinamide

Nicotinamide is also offered as part of the Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Nicotinamide

M. Wt	122.12
Formula	C₆H₆N₂O
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	98-92-0
PubChem ID	936
InChi Key	DFPAKSUCGFBDDF-UHFFFAOYSA-N
Smiles	NC(C1=CC=CN=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Nicotinamide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	12.21	100

Preparing Stock Solutions for Nicotinamide

The following data is based on the product molecular weight 122.12.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	8.19 mL	40.94 mL	81.89 mL
5 mM	1.64 mL	8.19 mL	16.38 mL
10 mM	0.82 mL	4.09 mL	8.19 mL
50 mM	0.16 mL	0.82 mL	1.64 mL

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Reconstitution Calculator

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References for Nicotinamide

References are publications that support the biological activity of the product.

Peled Nicotinamide, a SIRT1 inhibitor, inhibits differentiation and facilitates expansion of hematopoietic progenitor cells with enhanced bone marrow homing and engraftment. Exp.Hematol. 2012 PMID: 22198152
Jung Isolation and in vitro expansion of human colonic stem cells. Nat.Med. 2011 PMID: 21892181
Boj Organoid models of human and mouse ductal pancreatic cancer. Cell 2015 PMID: 25557080
Bartfeld In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection. Gastroenterology 2015 PMID: 25307862
Sato SnapShot: Growing organoids from stem cells. Cell 2015 PMID: 26091044
Czubak A modified method of Ins producing cells' generation from bone marrow-derived mesenchymal stem cells. J.Diabetes Res. 2014 PMID: 25405207
Cimadamore Nicotinamide rescues human embryonic stem cell-derived neuroectoderm from parthanatic cell death. Stem Cells. 2009 PMID: 19544437
Suzuki Protective effect of nicotinamide against poly(ADP-ribose) polymerase-1-mediated astrocyte death depends on its transporter-mediated uptake. Life.Sci. 2010 PMID: 20188745