SP 600125

Product Description

SP 600125 is a selective JNK inhibitor. Competitively and reversibly inhibits JNK1, 2 and 3 (IC₅₀ = 40 - 90 nM) with negligible activity at ERK2, p38β and a range of enzymes. Active in vivo. Exhibits reduced selectivity over other protein kinases under certain conditions. SP 600125 protects renal tubular epithelial cells from ischemia/reperfusion-induced apoptosis. Promotes chondrogenesis of bone mesenchymal stem cells by inhibition of TNF-α-induced inflammation. Adipogenesis from MSCs is also promoted by SP 600125. Essential component of medium for maintaining stem cells in naive pluripotent state.

Solubility

Preparing Stock Solutions for SP 600125

The following data is based on the product molecular weight 220.23

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.54 mL	22.70 mL	45.41 mL
5 mM	0.91 mL	4.54 mL	9.08 mL
10 mM	0.45 mL	2.27 mL	4.54 mL
50 mM	0.09 mL	0.45 mL	0.91 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Tominaga Negative regulation of adipogenesis from human mesenchymal stem cells by Jun N-terminal kinase. Biochem.Biophys.Res.Commun. 2005 PMID: 15582605
• Ding SP600125 restored TNF-α-induced impaired chondrogenesis in bone mesenchymal stem cells and its antiosteoarthritis effect in mice. Stem Cells Dev. 2021 PMID: 34486378
• Zhao Activation of JNKs is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells. BMB Rep. 2013 PMID: 23977991
• Wang SP600125, a selective JNK inhibitor, protects ischemic renal injury via suppressing the extrinsic pathways of apoptosis. Life Sci. 2007 PMID: 17459422
• Schnabl TAK1/JNK and p38 have opposite effects on rat hepatic stellate cells. Hepatology 2001 PMID: 11679966
• Bennett SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase. Proc.Natl.Acad.Sci.U.S.A. 2001 PMID: 11717429