Human Hematopoietic Progenitor Cell Multi-Color Flow Kit

R&D Systems, part of Bio-Techne | Catalog # FMC019

Contains conjugated antibodies to CD34-APC (Clone QBEnd10), CD38-PerCP (Clone 240742), CD11b-Fluorescein (Clone 238446), SCF R-PE (Clone 74233)
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Key Product Details

Species

Human

Conjugate

FITC, PE, APC

Product Summary for Human Hematopoietic Progenitor Cell Multi-Color Flow Kit

Kit Summary

To verify hematopoietic progenitor cell multipotency using 4-color flow cytometry.

Why is it Important to Verify Hematopoietic Progenitor Cell Identity using Multiple Established Markers?

Hematopoietic stem cells are multipotent, self-renewing progenitor cells from which all differentiated blood cell types arise during the process of hematopoiesis. Using multiple markers to characterize hematopoietic progenitors increases confidence in cell identity by clearly and consistently defining your target cell population.   See Details

In addition, the unique combination of negative and positive markers included in this kit decreases experimental variation in hematopoietic progenitor cell identification, resulting in an optimized starting cell population for your research.

  • Uses a combination of positive and negative cell-surface markers to verify progenitor cell identity
  • Verifies a healthy, multipotent starting progenitor cell population to increase consistency between studies and reduce unwanted experimental variability
  • Does not require the use of secondary antibodies

Kit Contents

This flow cytometry kit contains four fluorochrome-conjugated primary antibodies and corresponding isotype controls that can be used for single-step staining of human hematopoietic progenitor cells. This kit includes reagents sufficient for 25 tests.   See Details

  • Staining Buffer (100 mL)

Positive Markers:

  • APC-conjugated Mouse Anti-Human CD34 (Clone QBEnd10; Mouse IgG1)
  • PE-conjugated Mouse Anti-Human SCF R/CD117 (Clone 47233; Mouse IgG1)

Negative Markers:

  • PerCP-conjugated Mouse Anti-Human CD38 (Clone 240742; Mouse IgG2A)
  • CFS-conjugated Mouse Anti-Human CD11b (Clone 238446,IgG2B)

Positive Isotype Controls

  • APC-conjugated Mouse IgG1 Isotype Control (Clone 11711)
  • PE-conjugated Mouse IgG1 Isotype Control (Clone 11711)

Negative Isotype Controls

  • PerCP-conjugated Mouse IgG2A Isotype Control (Clone 20102)
  • CFS-conjugated Mouse IgG2B Isotype Control (Clone 133303)

Scientific Data Images for Human Hematopoietic Progenitor Cell Multi-Color Flow Kit

Identification of Human Hematopoietic Progenitor Cells using Multi-Color Flow Cytometry.

Identification of Human Hematopoietic Progenitor Cells using Multi-Color Flow Cytometry.

Human umbilical cord blood cells were stained using reagents supplied in this kit. Cells were simultaneously analyzed for the expression of multipotency markers. Cells negative for CD11b and negative/low for CD38 (boxed area in A) were gated and assessed for positive expression of CD34 and SCF R/CD117 (upper right quadrant in B). Quadrants were set based on isotype controls.
Identification of Human Hematopoietic Progenitor Cells using Multi-Color Flow Cytometry.

Identification of Human Hematopoietic Progenitor Cells using Multi-Color Flow Cytometry.

Human umbilical cord blood cells were stained with the indicated antibodies included in this kit. Representative expression of each analyte in the total umbilical cord blood cell population (filled histogram) over the isotype control (open histogram).

Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Hematopoietic Stem Cells

The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long mainte­nance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continu­ously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage deple­tion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.

Additional Hematopoietic Stem Cells Products

Product Documents for Human Hematopoietic Progenitor Cell Multi-Color Flow Kit

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Hematopoietic Progenitor Cell Multi-Color Flow Kit

For research use only

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