Mouse Oligodendrocyte Differentiation Kit

Kit Summary

A kit optimized to efficiently differentiate mouse pluripotent cells into oligodendrocytes under serum-free conditions.

Why Use Pre-optimized Reagents for Differentiation of Pluripotent Stem Cells into Oligodendrocytes?

Successful differentiation of pluripotent stem cells into oligodendrocytes is dependent on high quality media and differentiation supplements.

Oligodendrocytes are cells of the central nervous system which myelinate axons and support rapid nerve conduction. In CNS disorders, such as stroke, multiple sclerosis and spinal cord injury, demyelination of axons contributes to functional deficits. Studies have demonstrated that neural transplantation therapy can restore functions lost by demyelination. However, the use of neural transplantation is limited by an inadequate supply of oligodendrocyte precursors. R&D Systems offers the Mouse Oligodendrocyte Differentiation Kit to drive successful and efficient differentiation of embryonic and induced pluripotent stem cells into oligodendrocytes.

R&D Systems Mouse Oligodendrocyte Differentiation Kit:

• Is pre-optimized to reduce variation.
• Has defined components to decrease variation.
• Includes sufficient reagents for the differentiation of 3 x 10⁷ embryonic stem cells.

Kit Components

The Mouse Oligodendrocyte Differentiation Kit contains ITS and N-2 MAX Media Supplements, which are used to select and enrich neural precursor populations that are characterized by Nestin and A2B5 staining. Purified Bovine Fibronectin is included to support cell attachment and spreading. A growth factor panel, consisting of Recombinant Human Fibroblast Growth Factor Basic (FGF basic), Recombinant Human Epidermal Growth Factor (EGF), and Recombinant Human Platelet-derived Growth Factor AA (PDGF-AA) is included for effective oligodendrocyte differentiation.

The quantity of each component provided in the kit is estimated to be sufficient for the differentiation of 3 x 10⁷ embryonic stem cells.

• Recombinant Human EGF
• Recombinant Human FGF basic
• Recombinant Human PDGF-AA
• Bovine Fibronectin
• ITS Media Supplement
• N-2 MAX Media Supplement

Stability and Storage

• Unopened Kit should be stored at = -20 °C in a manual defrost freezer. Do not use the kit past the expiration date.
• Opened Reagents can be stored at 2 °C to 8 °C for up to 1 month or aliquotted and stored at = -20 °C in a manual defrost freezer for up to 6 months.* Avoid repeated freeze-thaw cycles.

*Provided this is within the expiration date of the kit.

Limitations of the Procedure

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR DIAGNOSTIC USE.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• The quality of the embryonic stem cells and any variation in this procedure may cause variation in the efficiency of oligodendrocyte generation.

Precautions

• The acute and chronic effects of overexposure to reagents of this kit are unknown. Safe laboratory procedures should be followed and protective clothing should be worn when handling kit reagents.
• The ITS and N-2 MAX Supplements contain human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, these reagents should be handled as if capable of transmitting infection.