Goat IgG HRP-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # HAF109
Key Product Details
Species Reactivity
Goat
Applications
Simple Western, Western Blot
Label
HRP
Antibody Source
Polyclonal Donkey IgG
Product Specifications
Immunogen
Goat IgG
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Scientific Data Images for Goat IgG HRP-conjugated Antibody
Detection of Goat IgG Primary Antibody by Western Blot
Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Polyclonal Antibody (Catalog # AF3584) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Goat IgG Primary Antibody by Simple WesternTM.
Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog # AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Goat IgG HRP-conjugated Antibody
Application
Recommended Usage
Simple Western
Use at a 1:50 dilution.
Western Blot
Use at a 1:1000 dilution.
Reviewed Applications
Read 4 reviews rated 4 using HAF109 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a 0.2 um filtered solution in PBS with stabilizers.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Goat IgG HRP-conjugated Antibody
Product Specific Notices for Goat IgG HRP-conjugated Antibody
For research use only
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